[HTML][HTML] CRISPR/Cas9-mediated genome editing techniques and new breeding strategies in cereals–current status, improvements, and perspectives
Cereal crops, including triticeae species (barley, wheat, rye), as well as edible cereals
(wheat, corn, rice, oat, rye, sorghum), are significant suppliers for human consumption …
(wheat, corn, rice, oat, rye, sorghum), are significant suppliers for human consumption …
Pyramiding for resistance durability: theory and practice
CC Mundt - Phytopathology, 2018 - Am Phytopath Society
Durable disease resistance is a key component of global food security, and combining
resistance genes into “pyramids” is an important way to increase durability of resistance. The …
resistance genes into “pyramids” is an important way to increase durability of resistance. The …
Transient Expression of CRISPR/Cas9 Machinery Targeting TcNPR3 Enhances Defense Response in Theobroma cacao
AS Fister, L Landherr, SN Maximova… - Frontiers in plant …, 2018 - frontiersin.org
Theobroma cacao, the source of cocoa, suffers significant losses to a variety of pathogens
resulting in reduced incomes for millions of farmers in developing countries. Development of …
resulting in reduced incomes for millions of farmers in developing countries. Development of …
CRISPR/Cas9-mediated efficient editing in phytoene desaturase (PDS) demonstrates precise manipulation in banana cv. Rasthali genome
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-
associated protein 9 (Cas9) has been reported for precise genome modification in many …
associated protein 9 (Cas9) has been reported for precise genome modification in many …
sgRNACNN: identifying sgRNA on-target activity in four crops using ensembles of convolutional neural networks
Key message We proposed an ensemble convolutional neural network model to identify
sgRNA high on-target activity in four crops and we used one-hot encoding and k-mers for …
sgRNA high on-target activity in four crops and we used one-hot encoding and k-mers for …
Potential high-frequency off-target mutagenesis induced by CRISPR/Cas9 in Arabidopsis and its prevention
Key message We present novel observations of high-specificity SpCas9 variants, sgRNA
expression strategies based on mutant sgRNA scaffold and tRNA processing system, and …
expression strategies based on mutant sgRNA scaffold and tRNA processing system, and …
CRISPR/Cas‐based tools for the targeted control of plant viruses
G Robertson, J Burger, M Campa - Molecular Plant Pathology, 2022 - Wiley Online Library
Plant viruses are known to infect most economically important crops and pose a major threat
to global food security. Currently, few resistant host phenotypes have been delineated, and …
to global food security. Currently, few resistant host phenotypes have been delineated, and …
Broadening the GMO risk assessment in the EU for genome editing technologies in agriculture
K Kawall, J Cotter, C Then - Environmental Sciences Europe, 2020 - Springer
Genome editing techniques, especially the CRISPR/Cas technology, increase the
possibilities and the speed of altering genetic material in organisms. So-called genome …
possibilities and the speed of altering genetic material in organisms. So-called genome …
CRISPR-Cas: A robust technology for enhancing consumer-preferred commercial traits in crops
The acceptance of new crop varieties by consumers is contingent on the presence of
consumer-preferred traits, which include sensory attributes, nutritional value, industrial …
consumer-preferred traits, which include sensory attributes, nutritional value, industrial …
Plant genome editing and the relevance of off-target changes
Site-directed nucleases (SDNs) used for targeted genome editing are powerful new tools to
introduce precise genetic changes into plants. Like traditional approaches, such as …
introduce precise genetic changes into plants. Like traditional approaches, such as …