[HTML][HTML] CRISPR/Cas9-mediated genome editing techniques and new breeding strategies in cereals–current status, improvements, and perspectives

S Ahmar, G Hensel, D Gruszka - Biotechnology Advances, 2023 - Elsevier
Cereal crops, including triticeae species (barley, wheat, rye), as well as edible cereals
(wheat, corn, rice, oat, rye, sorghum), are significant suppliers for human consumption …

Pyramiding for resistance durability: theory and practice

CC Mundt - Phytopathology, 2018 - Am Phytopath Society
Durable disease resistance is a key component of global food security, and combining
resistance genes into “pyramids” is an important way to increase durability of resistance. The …

Transient Expression of CRISPR/Cas9 Machinery Targeting TcNPR3 Enhances Defense Response in Theobroma cacao

AS Fister, L Landherr, SN Maximova… - Frontiers in plant …, 2018 - frontiersin.org
Theobroma cacao, the source of cocoa, suffers significant losses to a variety of pathogens
resulting in reduced incomes for millions of farmers in developing countries. Development of …

CRISPR/Cas9-mediated efficient editing in phytoene desaturase (PDS) demonstrates precise manipulation in banana cv. Rasthali genome

N Kaur, A Alok, null Shivani, N Kaur, P Pandey… - Functional & integrative …, 2018 - Springer
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-
associated protein 9 (Cas9) has been reported for precise genome modification in many …

sgRNACNN: identifying sgRNA on-target activity in four crops using ensembles of convolutional neural networks

M Niu, Y Lin, Q Zou - Plant molecular biology, 2021 - Springer
Key message We proposed an ensemble convolutional neural network model to identify
sgRNA high on-target activity in four crops and we used one-hot encoding and k-mers for …

Potential high-frequency off-target mutagenesis induced by CRISPR/Cas9 in Arabidopsis and its prevention

Q Zhang, HL Xing, ZP Wang, HY Zhang, F Yang… - Plant molecular …, 2018 - Springer
Key message We present novel observations of high-specificity SpCas9 variants, sgRNA
expression strategies based on mutant sgRNA scaffold and tRNA processing system, and …

CRISPR/Cas‐based tools for the targeted control of plant viruses

G Robertson, J Burger, M Campa - Molecular Plant Pathology, 2022 - Wiley Online Library
Plant viruses are known to infect most economically important crops and pose a major threat
to global food security. Currently, few resistant host phenotypes have been delineated, and …

Broadening the GMO risk assessment in the EU for genome editing technologies in agriculture

K Kawall, J Cotter, C Then - Environmental Sciences Europe, 2020 - Springer
Genome editing techniques, especially the CRISPR/Cas technology, increase the
possibilities and the speed of altering genetic material in organisms. So-called genome …

CRISPR-Cas: A robust technology for enhancing consumer-preferred commercial traits in crops

V Verma, A Kumar, M Partap, M Thakur… - Frontiers in Plant …, 2023 - frontiersin.org
The acceptance of new crop varieties by consumers is contingent on the presence of
consumer-preferred traits, which include sensory attributes, nutritional value, industrial …

Plant genome editing and the relevance of off-target changes

N Graham, GB Patil, DM Bubeck, RC Dobert… - Plant …, 2020 - academic.oup.com
Site-directed nucleases (SDNs) used for targeted genome editing are powerful new tools to
introduce precise genetic changes into plants. Like traditional approaches, such as …