Natto, a fermented soybean product, has been consumed as a traditional food in Japan for
thousands of years. Nattokinase (NK), a potent blood-clot dissolving protein used for the …

Nattokinase (NK, also known as subtilisin NAT)(EC 3.4. 21.62) is one of the most
considerable extracellular enzymes produced by Bacillus subtilis natto. The main interest …

The well-characterized gram-positive bacterium Bacillus subtilis is an outstanding industrial
candidate for protein expression owing to its single membrane and high capacity of …

Using double promoter expression systems is a promising approach to increase
heterologous protein production. In this review, current double promoter expression systems …

Background Nattokinase (NK), which is a member of the subtilisin family, is a potent
fibrinolytic enzyme that might be useful for thrombosis therapy. Extensive work has been …

The manno endo-1, 4-mannosidase (β-mannanase, EC. 3.2. 1.78) catalyzes the random
hydrolysis of internal (1→ 4)-β-mannosidic linkages in the mannan polymers. A codon …

Nattokinase (NK) possesses the potential for prevention and treatment of thrombus-related
diseases. In this study, high-level expression of nattokinase was achieved in Bacillus …

Abstract Background Bacillus subtilis, a Gram-positive organism, has been developed to be
an attractive expression platform to produce both secreted and cytoplasmic proteins owing …

Background Bacillus subtilis is developed to be an attractive expression host to produce
both secreted and cytoplasmic proteins owing to its prominent biological characteristics …

Bacillus subtilis is an attractive host for production of recombinant proteins. Promoters and
expression plasmid backbones have direct impacts on the efficiency of gene expression. To …