The traditional two-dimensional (2D) in vitro cell culture system (on a flat support) has long
been used in cancer research. However, this system cannot be fully translated into clinical …

In comparison with 2D cell culture models, 3D spheroids are able to accurately mimic some
features of solid tumors, such as their spatial architecture, physiological responses …

In previous years, three‑dimensional (3D) cell culture technology has become a focus of
research in tumor cell biology, using a variety of methods and materials to mimic the in vivo …

Highlights•Comprehensive review of recent advances in 3D bioprinting techniques for tissue
engineering.•3D bioprinting provides complex 3D biomimetic architectures for 3D cell …

To curb the high cost of drug development, there is an urgent need to develop more
predictive tissue models using human cells to determine drug efficacy and safety in advance …

The preclinical drug screening of pancreatic cancer treatments suffers from the absence of
appropriate models capable to reproduce in vitro the heterogeneous tumor …

The basement membrane is an important extracellular matrix that is found in all epithelial
and endothelial tissues. It maintains tissue integrity, serves as a barrier to cells and to …

Simple Summary Presently, the investigation of the tumor is carried out using different
models. One of the promising areas is the creation of 3D tumor models, such as spheroids …

Cancers are complex and heterogeneous pathological “organs” in a dynamic interplay with
their host. Models of human cancer in vitro, used in cancer biology and drug discovery, are …

This review covers several aspects of microfluidic devices used for culturing and monitoring
of both adherent and non-adherent cells, including a multitude of applications. A comparison …