Cells throughout nature contain multiple and overlapping DNA repair pathways that are
essential for maintaining the integrity of genomic DNA. 1, 2 The DNA repair pathway known …

DNA polymerases are molecular motors directing the synthesis of DNA from nucleotides. All
polymerases have a common architectural framework consisting of three canonical …

The X family of DNA polymerases in eukaryotic cells consists of terminal transferase, and
DNA polymerases β, λ, and μ. These enzymes have similar structural portraits, yet different …

To dissect the effects of the nucleotide-binding and catalytic metal ions on DNA polymerase
mechanisms for DNA repair and synthesis, aside from the chemical reaction, we investigate …

Our application of transition path sampling to a complex biomolecular system in explicit
solvent, the closing transition of DNA polymerase β, unravels atomic and energetic details of …

Various kinetic studies on nucleotide incorporation by DNA polymerases have established
that a rate-limiting step occurs that is crucial in the mechanism of discrimination between …

Pre-steady-state kinetic analysis was used to compare the catalytic properties of DNA
polymerase β (Pol β) for single-base gap-filling and regular duplex DNA synthesis. The rate …

Stopped-flow fluorescence assay was applied to identify conformational changes in the
catalytic cycle of DNA polymerase β (Pol β), using a synthetic DNA primer/template …

To understand how the active site of a DNA polymerase might modulate the coding of 8-oxo-
7, 8-dihydrodeoxyguanine (8-oxodG), we performed steady-state kinetic analyses using wild …

X-ray crystallographic structures of human DNA polymerase β with nonhydrolyzable analogs
containing all atoms in the active site required for catalysis provide a secure starting point for …