Analysis and visualization of quantitative proteomics data using FragPipe-Analyst

Y Hsiao, H Zhang, GX Li, Y Deng, F Yu… - Journal of Proteome …, 2024 - ACS Publications
The FragPipe computational proteomics platform is gaining widespread popularity among
the proteomics research community because of its fast processing speed and user-friendly …

Global, in situ analysis of the structural proteome in individuals with Parkinson's disease to identify a new class of biomarker

MT Mackmull, L Nagel, F Sesterhenn… - Nature structural & …, 2022 - nature.com
Parkinson's disease (PD) is a prevalent neurodegenerative disease for which robust
biomarkers are needed. Because protein structure reflects function, we tested whether …

MS-DAP platform for downstream data analysis of label-free proteomics uncovers optimal workflows in benchmark data sets and increased sensitivity in analysis of …

F Koopmans, KW Li, RV Klaassen… - Journal of Proteome …, 2022 - ACS Publications
In the rapidly moving proteomics field, a diverse patchwork of data analysis pipelines and
algorithms for data normalization and differential expression analysis is used by the …

Systematic identification of 20S proteasome substrates

M Pepelnjak, R Rogawski, G Arkind… - Molecular Systems …, 2024 - embopress.org
For years, proteasomal degradation was predominantly attributed to the ubiquitin-26S
proteasome pathway. However, it is now evident that the core 20S proteasome can …

In situ analysis of osmolyte mechanisms of proteome thermal stabilization

M Pepelnjak, B Velten, N Näpflin, T von Rosen… - Nature Chemical …, 2024 - nature.com
Organisms use organic molecules called osmolytes to adapt to environmental conditions. In
vitro studies indicate that osmolytes thermally stabilize proteins, but mechanisms are …

prolfqua: A Comprehensive R-Package for Proteomics Differential Expression Analysis

WE Wolski, P Nanni, J Grossmann… - Journal of Proteome …, 2023 - ACS Publications
Mass spectrometry is widely used for quantitative proteomics studies, relative protein
quantification, and differential expression analysis of proteins. There is a large variety of …

Structures of wild-type and selected CMT1X mutant connexin 32 gap junction channels and hemichannels

C Qi, P Lavriha, E Bayraktar, A Vaithia, D Schuster… - Science …, 2023 - science.org
In myelinating Schwann cells, connection between myelin layers is mediated by gap
junction channels (GJCs) formed by docked connexin 32 (Cx32) hemichannels (HCs) …

A chemical probe to modulate human GID4 Pro/N-degron interactions

DDG Owens, MER Maitland, A Khalili Yazdi… - Nature Chemical …, 2024 - nature.com
The C-terminal to LisH (CTLH) complex is a ubiquitin ligase complex that recognizes
substrates with Pro/N-degrons via its substrate receptor Glucose-Induced Degradation 4 …

Comprehensive overview of bottom-up proteomics using mass spectrometry

Y Jiang, DAB Rex, D Schuster, BA Neely… - ACS Measurement …, 2024 - ACS Publications
Proteomics is the large scale study of protein structure and function from biological systems
through protein identification and quantification.“Shotgun proteomics” or “bottom-up …

Mass Spectrometry Structural Proteomics Enabled by Limited Proteolysis and Cross‐Linking

H Lu, Z Zhu, L Fields, H Zhang… - Mass Spectrometry …, 2024 - Wiley Online Library
The exploration of protein structure and function stands at the forefront of life science and
represents an ever‐expanding focus in the development of proteomics. As mass …