Gene expression occurs in two essential steps: transcription and translation. In bacteria, the
two processes are tightly coupled in time and space, and highly regulated. Tight regulation …

mRNA degradation is a central process that affects all gene expression levels, and yet, the
determinants that control mRNA decay rates remain poorly characterized. Here, we applied …

Synthetic biological circuits are promising tools for developing sophisticated systems for
medical, industrial, and environmental applications. So far, circuit implementations …

Identifying the sources of cell-to-cell variability in signaling dynamics is essential to
understand drug response variability and develop effective therapeutics. However, it is …

Synthetic biology has been instrumental in turning microbes into cell-factories capable of
diverse processes. A recent application has been to convert them into living therapeutics …

Motivation Cell function is regulated by gene regulatory networks (GRNs) defined by protein-
mediated interaction between constituent genes. Despite advances in experimental …

To build complex genetic networks with predictable behaviors, synthetic biologists use
libraries of modular parts that can be characterized in isolation and assembled together to …

RNA-based devices controlling gene expression bear great promise for synthetic biology, as
they offer many advantages such as short response times and light metabolic burden …

Motivation Advances in experimental and imaging techniques have allowed for
unprecedented insights into the dynamical processes within individual cells. However, many …

RNA-based regulators are promising tools for building synthetic biological systems that
provide a powerful platform for achieving a complex regulation of transcription and …