[PDF][PDF] The potential of high resolution melting analysis (hrma) to streamline, facilitate and enrich routine diagnostics in medical microbiology.

L Ruskova, V Raclavsky - … Papers of the Medical Faculty of …, 2011 - biomed.papers.upol.cz
Background. Routine medical microbiology diagnostics relies on conventional cultivation
followed by phenotypic techniques for identification of pathogenic bacteria and fungi. This is …

[HTML][HTML] The β-tubulin gene as a molecular phylogenetic marker for classification and discrimination of the Saccharomyces sensu stricto complex

CH Huang, FL Lee, CJ Tai - Antonie Van Leeuwenhoek, 2009 - Springer
The Saccharomyces sensu stricto complex comprises seven very closely related species. In
this study, we compared the use of two different phylogenetic markers, the 26S rDNA and β …

Candida “psilosis”–electromigration techniques and MALDI-TOF mass spectrometry for phenotypical discrimination

A Kubesová, J Šalplachta, M Horká, F Růžička, K Šlais - Analyst, 2012 - pubs.rsc.org
Biofilm-positive strains of Candida parapsilosis are the second most common yeasts
responsible for bloodstream infections. This pathogen is difficult to identify by standard …

[引用][C] 侵袭性真菌感染实验室早期诊断方法的现状与展望

曹楠楠, 郑磊, 王前 - 国际检验医学杂志, 2011

High-resolution melt-curve analysis of random-amplified-polymorphic-DNA markers, for the characterisation of pathogenic Leptospira

SM Tulsiani, SB Craig, GC Graham… - Annals of Tropical …, 2010 - Taylor & Francis
A new test for pathogenic Leptospira isolates, based on RAPD–PCR and high-resolution
melt (HRM) analysis (which measures the melting temperature of amplicons in real time …

Separation of phenotypically indistinguishable Candida species, C. orthopsilosis, C. metapsilosis and C. parapsilosis, by capillary electromigration techniques

M Horká, F Růžička, A Kubesová, E Němcová… - … of Chromatography A, 2011 - Elsevier
At the current state of laboratory diagnostics, methods for fast identification of phenotypically
indistinguishable species are difficult or inaccurate. An example is represented by Candida …

[HTML][HTML] Performance of optimized McRAPD in identification of 9 yeast species frequently isolated from patient samples: potential for automation

J Trtkova, P Pavlicek, L Ruskova, P Hamal… - BMC microbiology, 2009 - Springer
Background Rapid, easy, economical and accurate species identification of yeasts isolated
from clinical samples remains an important challenge for routine microbiological …

[HTML][HTML] An interlaboratory comparison of ITS2-PCR for the identification of yeasts, using the ABI Prism 310 and CEQ8000 capillary electrophoresis systems

T De Baere, A Van Keerberghen, P Van Hauwe… - BMC microbiology, 2005 - Springer
Background Currently, most laboratories identify yeasts routinely on the basis of morphology
and biochemical reactivity. This approach has quite often limited discriminatory power and …

Usefulness of McRAPD for typing and importance of biofilm production in a case of nosocomial ventriculoperitoneal shunt infection caused by Candida lusitaniae

P Hamal, J Hanzen, F Horn, J Trtkova, L Ruskova… - Folia microbiologica, 2011 - Springer
A case report of ventriculoperitoneal shunt infection caused by Candida lusitaniae in a 6-
year-old patient with cerebral astrocytoma and obstructive hydrocephalus is presented …

[引用][C] RAPD 技术在酿酒酵母分类鉴定与遗传育种中的应用

杨智, 杨振泉, 汪志君, 方维明 - 现代食品科技, 2007