Haematopoietic stem cell self-renewal in vivo and ex vivo
AC Wilkinson, KJ Igarashi, H Nakauchi - Nature Reviews Genetics, 2020 - nature.com
The self-renewal capacity of multipotent haematopoietic stem cells (HSCs) supports blood
system homeostasis throughout life and underlies the curative capacity of clinical HSC …
system homeostasis throughout life and underlies the curative capacity of clinical HSC …
A high-fidelity Cas9 mutant delivered as a ribonucleoprotein complex enables efficient gene editing in human hematopoietic stem and progenitor cells
Translation of the CRISPR–Cas9 system to human therapeutics holds high promise.
However, specificity remains a concern especially when modifying stem cell populations …
However, specificity remains a concern especially when modifying stem cell populations …
[HTML][HTML] Cas9-AAV6 gene correction of beta-globin in autologous HSCs improves sickle cell disease erythropoiesis in mice
Abstract CRISPR/Cas9-mediated beta-globin (HBB) gene correction of sickle cell disease
(SCD) patient-derived hematopoietic stem cells (HSCs) in combination with autologous …
(SCD) patient-derived hematopoietic stem cells (HSCs) in combination with autologous …
[HTML][HTML] Hematopoietic stem cell gene editing and expansion: State-of-the-art technologies and recent applications
Hematopoietic stem cell transplantation (HSCT) is a curative therapy for a range of
hematological diseases, from leukemias to immunodeficiencies and anemias. The aim in …
hematological diseases, from leukemias to immunodeficiencies and anemias. The aim in …
Engineering resistance to CD33-targeted immunotherapy in normal hematopoiesis by CRISPR/Cas9-deletion of CD33 exon 2
CD33 has long been pursued as immunotherapeutic target in acute myeloid leukemia
(AML)[1, 2]. Improved survival with gemtuzumab ozogamicin (GO) validates this approach …
(AML)[1, 2]. Improved survival with gemtuzumab ozogamicin (GO) validates this approach …
[HTML][HTML] Lineage commitment of hematopoietic stem cells and progenitors: insights from recent single cell and lineage tracing technologies
Highlights•Cellular lineage commitment within hematopoiesis is reviewed.•Insights from
novel lineage tracing and single-cell transcriptomics studies are discussed.•How single cell …
novel lineage tracing and single-cell transcriptomics studies are discussed.•How single cell …
Stabilizing hematopoietic stem cells in vitro
AC Wilkinson, H Nakauchi - Current opinion in genetics & development, 2020 - Elsevier
Hematopoietic stem cells (HSCs) can regenerate all lineages of the adult blood and immune
systems long-term following transplantation via a combination of self-renewal and …
systems long-term following transplantation via a combination of self-renewal and …
[HTML][HTML] Decreased eggshell strength caused by impairment of uterine calcium transport coincide with higher bone minerals and quality in aged laying hens
Y Fu, J Zhou, M Schroyen, H Zhang, S Wu, G Qi… - Journal of Animal …, 2024 - Springer
Background Deteriorations in eggshell and bone quality are major challenges in aged
laying hens. This study compared the differences of eggshell quality, bone parameters and …
laying hens. This study compared the differences of eggshell quality, bone parameters and …
[HTML][HTML] CRISPR/Cas-Based gene editing strategies for DOCK8 immunodeficiency syndrome
S Ravendran, SS Hernández, S König… - Frontiers in Genome …, 2022 - frontiersin.org
Defects in the DOCK8 gene causes combined immunodeficiency termed DOCK8
immunodeficien-cy syndrome (DIDS). DIDS previously belonged to the disease category of …
immunodeficien-cy syndrome (DIDS). DIDS previously belonged to the disease category of …
[HTML][HTML] Single-cell sequencing in normal and malignant hematopoiesis
NK Wilson, B Göttgens - HemaSphere, 2018 - journals.lww.com
Hematopoiesis is one of the best studied adult stem-cell systems, with a differentiation
hierarchy progressing from immature hematopoietic stem cells to over 10 distinct mature cell …
hierarchy progressing from immature hematopoietic stem cells to over 10 distinct mature cell …