Establishment of targeted mutagenesis in soybean protoplasts using CRISPR/Cas9 RNP delivery via electro− transfection

S Subburaj, SZ Agapito-Tenfen - Frontiers in Plant Science, 2023 - frontiersin.org
The soybean (Glycine max L.) is an important crop with high agronomic value. The
improvement of agronomic traits through gene editing techniques has broad application …

A comprehensive review of in planta stable transformation strategies

JG Bélanger, TR Copley, V Hoyos-Villegas, JB Charron… - Plant Methods, 2024 - Springer
Plant transformation remains a major bottleneck to the improvement of plant science, both
on fundamental and practical levels. The recalcitrant nature of most commercial and minor …

Development of virus-induced genome editing methods in Solanaceous crops

SY Lee, B Kang, J Venkatesh, JH Lee… - Horticulture …, 2024 - academic.oup.com
Genome editing (GE) using CRISPR/Cas systems has revolutionized plant mutagenesis.
However, conventional transgene-mediated GE methods have limitations due to the time …

Applications of Virus-Induced Gene Silencing in Cotton

Y Tian, Y Fang, K Zhang, Z Zhai, Y Yang, M He, X Cao - Plants, 2024 - mdpi.com
Virus-induced gene silencing (VIGS) is an RNA-mediated reverse genetics technique that
has become an effective tool to investigate gene function in plants. Cotton is one of the most …

Trans‐complementation of the viral movement protein mediates efficient expression of large target genes via a tobacco mosaic virus vector

W Huang, Y Zhang, N Xiao, W Zhao… - Plant Biotechnology …, 2024 - Wiley Online Library
The development of plant virus‐based expression systems has expanded rapidly owing to
their potential applications in gene functional and disease resistance research, and …

Targeted genome editing for cotton improvement: prospects and challenges

R Kumar, J Das, RK Puttaswamy, M Kumar… - The Nucleus, 2024 - Springer
Cotton is a highly profitable agricultural crop globally, valued for its valuable natural
cellulosic fibres, renewable biofuel potential, and rich edible oil content. Global cotton …

Exploiting viral vectors to deliver genome editing reagents in plants

Y Shen, T Ye, Z Li, TH Kimutai, H Song, X Dong, J Wan - aBIOTECH, 2024 - Springer
Genome editing holds great promise for the molecular breeding of plants, yet its application
is hindered by the shortage of simple and effective means of delivering genome editing …

病毒介导的植物基因组编辑技术研究进展

胡丹玲, 孙永伟 - 植物学报, 2024 - chinbullbotany.com
作为一种新兴的靶向基因组编辑技术, 具有操作过程简便, 编辑效率高和支持多靶点编辑等优势,
在植物遗传育种中展现了广阔的应用前景. 然而对于一些遗传转化体系和再生体系尚未成熟的 …

Efficient tobacco rattle virus‐induced gene editing in tomato mediated by the CRISPR/Cas9 system

Q Wang, D Zhang, YR Dai, CC Liu - Biotechnology Journal, 2024 - Wiley Online Library
Plant virus‐based sgRNA delivery strategy has been widely applied for efficient genome
editing across various plant species, leveraging its significant advantages in the rapid …

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Associated Proteins (Cas)[CRISPR–Cas]: An Emerging Technique in Plant Disease Detection …

SK Ghosh, T Chatterjee - Gene Editing in Plants: CRISPR-Cas and Its …, 2024 - Springer
A great challenge has come to scientists to provide food to every person of the growing
population that will reach nearly ten billion globally within 2050. Another challenge for us is …