Highlights•Kinetoplastids do not control transcription of individual protein-coding
genes.•mRNAs are made from polycistronic precursors by trans splicing and …

Human visceral leishmaniasis (HVL) is the most severe clinical form of a spectrum of
neglected tropical diseases caused by protozoan parasites of the genus Leishmania …

Trypanosoma brucei evades the adaptive immune response through the expression of
antigenically distinct Variant Surface Glycoprotein (VSG) coats. To understand the …

While gene expression is a fundamental and tightly controlled cellular process that is
regulated at multiple steps, the exact contribution of each step remains unknown in any …

Protein abundance differs from a few to millions of copies per cell. Trypanosoma brucei
presents an excellent model for studies on codon bias and differential gene expression …

Background Chronic chagasic cardiomyopathy is a debilitating and frequently fatal outcome
of human infection with the protozoan parasite, Trypanosoma cruzi. Microarray analysis of …

A major obstacle to reproducible expression of recombinant transcripts lies in the epigenetic
effects of the flanking chromatin following integration. We previously presented a strategy to …

Here, we present a simple modular extendable vector system for introducing the T7 RNA
polymerase and tetracycline repressor genes into Trypanosoma brucei. This novel system …

The genetic code is an abstraction of how mRNA codons and tRNA anticodons molecularly
interact during protein synthesis; the stability and regulation of this interaction remains …

Whether codon usage fine-tunes mRNA translation in mammals remains controversial, with
recent papers suggesting that production of proteins in specific Gene Ontological (GO) …