Engineering, applications, and future perspectives of GPCR‐based genetically encoded fluorescent indicators for neuromodulators

VL Rohner, PJ Lamothe‐Molina… - Journal of …, 2024 - Wiley Online Library
This review explores the evolving landscape of G‐protein‐coupled receptor (GPCR)‐based
genetically encoded fluorescent indicators (GEFIs), with a focus on their development …

[HTML][HTML] Central amygdala CRF+ neurons promote heightened threat reactivity following early life adversity in mice

C Demaestri, M Pisciotta, N Altunkeser, G Berry… - Nature …, 2024 - nature.com
Failure to appropriately predict and titrate reactivity to threat is a core feature of fear and
anxiety-related disorders and is common following early life adversity (ELA). A population of …

Cell signaling in the circadian pacemaker: new insights from in vivo imaging

X Bonnefont - Neuroendocrinology, 2024 - karger.com
Background:“One for all, and all for one,” the famous rallying cry of the Three Musketeers, in
Alexandre Dumas's popular novel, certainly applies to the 20,000 cells composing the …

[HTML][HTML] Dopamine neurons drive spatiotemporally heterogeneous striatal dopamine signals during learning

L Engel, AR Wolff, M Blake, VL Collins, S Sinha… - bioRxiv, 2023 - ncbi.nlm.nih.gov
Environmental cues, through Pavlovian learning, become conditioned stimuli that invigorate
and guide animals toward acquisition of rewards. Dopamine neurons in the ventral …

[HTML][HTML] Protocol for fiber photometry recording from deep brain regions in head-fixed mice

S Zhou, S Duan, H Yang - STAR protocols, 2024 - Elsevier
To exclude the influence of motion on in vivo calcium imaging, animals usually need to be
fixed. However, the whole-body restraint can cause stress in animals, affecting experimental …

[HTML][HTML] Recording the Brain in Vivo: Emerging Technologies for the Exploration of Mental Health Conditions: Mounting interest in mental health conditions over the …

T Free - BioTechniques, 2024 - Taylor & Francis
Recording the brain in vivo: emerging technologies for the exploration of mental health
conditions | BioTechniques Future Science Logo Search This Journal Anywhere Quick Search …

An integrated microfluidic and fluorescence platform for probing in vivo neuropharmacology

SC Piantadosi, MK Lee, M Wu, H Huynh, R Avila… - bioRxiv, 2024 - biorxiv.org
Neurotechnologies and genetic tools for dissecting neural circuit functions have advanced
rapidly over the past decade, although the development of complementary pharmacological …

Depth-resolved fibre photometry for Alzheimer's disease plaque pathology in vivo

N Byron - 2024 - stax.strath.ac.uk
The progression of potential Alzheimer's disease (AD) treatments to the clinic may be
hindered by the pre-clinical screening approach used to access plaque-modifying effects in …

Automated device for simultaneous photometry and electrophysiology in freely moving animals

B De Corte, Y Kim, K Heslin, J Freeman, E Simpson… - 2024 - researchsquare.com
Photometry and electrophysiology are powerful tools for investigating brain-behavior
relationships. Combining these techniques would allow us to ask previously un-addressable …

Obtaining artifact-corrected signals in fiber photometry: Isosbestic signals, robust regression and dF/F calculations

LJ Keevers, GP McNally, P Jean-Richard-dit-Bressel - 2024 - researchsquare.com
Fiber photometry is a powerful tool to measure a wide variety of brain dynamics from
targeted cells and circuits in freely-behaving animals. However, measured biosensor signals …