Unmethylated CpG motifs are prevalent in bacterial but not vertebrate genomic DNAs.
Oligodeoxynucleotides (ODN) containing CpG motifs activate host defense mechanisms …

Gene delivery using nonviral approaches has been extensively studied as a basic tool for
intracellular gene transfer and gene therapy. In the past, the primary focus has been on …

Toll‐like receptors (TLRs) detect infections by highly conserved components of pathogens
that are either not present in our own cells or are normally sequestered in cellular …

▪ Abstract Owing to the rapid development of in vivo applications for non-viral gene delivery
vectors, it is necessary to have a better understanding of how the structure-activity …

Gene therapy promises to revolutionize medicine by treating the causes of disease rather
than the symptoms. We are nearing the end of the first decade of gene therapy, and this …

The past several years have witnessed a rapidly increasing number of reports on utilizing
plasmid DNA as a vector for the introduction of genes into mammalian cells for use in both …

Critical molecular and cellular biological factors impacting design of licensable DNA vaccine
vectors that combine high yield and integrity during bacterial production with increased …

Inflammatory toxicity represents a typical toxicity associated with systemic administration of
cationic liposome/DNA complex (lipoplex). Collected information indicates that the lipoplex …

It has become apparent that the clinical success anticipated in the field of gene therapy has
been limited by progress in several of the fundamental areas of genetics, molecular and …

Poor solubility of polycation complexes with DNA is one drawback for their in vivo use as
gene delivery systems. PEGylation often can improve the solubility of the complexes …