Abstract The Rho GTPases—RHOA, RAC1 and CDC42—are small GTP binding proteins
that regulate basic biological processes such as cell locomotion, cell division and …

Abstract Small GTPases including Ras, Rho, Rab, Arf, and Ran are omnipresent molecular
switches in regulating key cellular functions. Their dysregulation is a therapeutic target for …

How can a self-organized cellular function evolve, adapt to perturbations, and acquire new
sub-functions? To make progress in answering these basic questions of evolutionary cell …

Accurate decoding of spatial chemical landscapes is critical for many cell functions.
Eukaryotic cells decode local chemical gradients to orient growth or movement in productive …

In this article, we provide detailed protocols on using optogenetic dimerizers to acutely
perturb activities of guanine nucleotide exchange factors (GEFs) specific to Ras, Rac or Rho …

Schizosaccharomyces pombe is a widely used model organism to study many aspects of
eukaryotic cell physiology. Its popularity as an experimental system partially stems from the …

Optogenetic tools are created to control RhoA GTPase, a central regulator of actin
organization and actomyosin contractility. RhoA GTPase, or its upstream activator …

Polycystins are a family of conserved ion channels, mutations of which lead to one of the
most common human genetic disorders, namely, autosomal dominant polycystic kidney …

Cells self-organize using reaction-diffusion and fluid-flow principles. Whether bulk
membrane flows contribute to cell patterning has not been established. Here, using …

Cell division control protein 42 homolog (Cdc42) protein, a Ras superfamily GTPase,
regulates cellular activities, including cancer progression. Using all-atom molecular …