Human DNA extraction from highly degraded skeletal remains: How to find a suitable method?

DC Vinueza‐Espinosa, C Santos… - …, 2020 - Wiley Online Library
Retrieving DNA from highly degraded human skeletal remains is still a challenge due to low
concentration and fragmentation, which makes it difficult to extract and purify. Recent works …

Comparison of two methods for isolating DNA from human skeletal remains for STR analysis

C Rucinski, AL Malaver, EJ Yunis… - Journal of Forensic …, 2012 - Wiley Online Library
The quality and efficiency of a standard organic DNA isolation method and a silica‐based
method using the QIAGEN Blood Maxi Kit were compared to obtain human DNA and short …

Kinship analysis of skeletal remains from the Middle Ages

M Dzehverovic, B Jusic, A Pilav, T Lukic… - Forensic Science …, 2023 - Elsevier
Abstract Medieval cemeteries Klisa-Guca Gora, Alihodze and Glavica-Han Bila located in
the Travnik area (Travnik, Bosnia and Herzegovina) were archaeologically examined in the …

Quantitative analysis of DNA degradation in the dead body

M Itani, Y Yamamoto, S Miyaishi - Acta Medica …, 2011 - ousar.lib.okayama-u.ac.jp
Postmortem degradation of DNA was quantitatively estimated. Brain, liver, kidney and
muscle samples were obtained from sacrificed rats left at 20℃ or 4℃. The quantity of DNA …

Testis-specific protein Y-encoded copy number is correlated to its expression and the field fertility of Canadian Holstein bulls

CK Hamilton, AR Verduzco-Gómez, LA Favetta… - Sexual …, 2012 - karger.com
Testis-specific protein Y-encoded (TSPY) is present in varying copy number in both human
(20–76 copies) and cattle (37–200 copies), and some studies have linked this variation to …

Evaluation of different methods for DNA extraction from human burnt bones and the generation of genetic profiles for identification

A Uzair, N Rasool, M Wasim - Medicine, Science and the …, 2017 - journals.sagepub.com
Bone exposure to heat in the presence of moisture breaks the phosphodiester bonds of the
backbone, leaving sheared DNA in bone cells. This also limits the possibility of generating a …

Organic extraction of bone lysates improves DNA purification with silica beads

S Desmyter, G De Cock, S Moulin, F Noël - Forensic science international, 2017 - Elsevier
In our standard protocol for DNA extraction from skeletal remains of unidentified bodies,
bone lysates resulting from decalcification and Proteinase K treatment were purified with the …

Getting ahead: Extraction of DNA from skeletonized cranial material and teeth

SM Edson - Journal of forensic sciences, 2019 - Wiley Online Library
Between 1990 and 2018, the Defense POW/MIA Accounting Agency submitted 2177 cranial
elements and 1565 teeth to the Armed Forces Medical Examiner System—Armed Forces …

Identification of missing norwegian world war II soldiers, in Karelia Russia

I Morild, SS Hamre, R Huel… - Journal of forensic …, 2015 - Wiley Online Library
This article presents the multidisciplinary effort in trying to identify the skeletal remains of 100
N orwegian soldiers serving in the G erman army, killed in K arelia R ussia in 1944, from the …

[HTML][HTML] Procedures to recover DNA from pre-molar and molar teeth of decomposed cadavers with different post-mortem intervals

PE Raimann, JB Picanço, DSBS Silva… - Archives of Oral …, 2012 - Elsevier
A task-force to resolve 26 pending forensic caseworks was carried out. We tested four
different protocols to extract DNA from molar and pre-molar teeth from 26 cadavers with post …