Proteomic profiling of cellular stresses in Bacillus subtilis reveals cellular networks and assists in elucidating antibiotic mechanisms of action

JE Bandow, M Hecker - Systems Biological Approaches in Infectious …, 2007 - Springer
Proteomic profiling provides a global view of the protein composition of the cell. In contrast to
the static nature of the genome sequence, which provides the blueprint for all protein-based …

Control of the Phosphorylation State of the HPr Protein of the Phosphotransferase System in Bacillus subtilis: Implication of the Protein Phosphatase PrpC

KD Singh, S Halbedel, B Görke, J Stülke - Journal of Molecular …, 2007 - karger.com
In the Gram-positive bacterium Bacillus subtilis as well as in other firmicutes, the HPr protein
of the phosphotransferase system (PTS) has two distinct phosphorylation sites, His-15 and …

[HTML][HTML] Identification on membrane and characterization of phosphoproteins using an alkoxide-bridged dinuclear metal complex as a phosphate-binding tag …

T Nakanishi, E Ando, M Furuta, E Kinoshita… - Journal of …, 2007 - ncbi.nlm.nih.gov
We have developed a method for on-membrane direct identification of phosphoproteins,
which are detected by a phosphate-binding tag (Phos-tag) that has an affinity to phosphate …

A comparative proteomic approach to understand the adaptations of an H+‐ATPase‐defective mutant of Corynebacterium glutamicum ATCC14067 to energy …

L Li, M Wada, A Yokota - Proteomics, 2007 - Wiley Online Library
Abstract F172‐8, an H+‐ATPase‐defective mutant of the glutamic acid‐producing bacterium
Corynebacterium glutamicum ATCC 14067, exhibits enhanced rates of glucose …

Transcriptional activator YesS is stimulated by histidine-phosphorylated HPr of the Bacillus subtilis phosphotransferase system

S Poncet, M Soret, P Mervelet, J Deutscher… - Journal of Biological …, 2009 - ASBMB
In low GC content Gram-positive bacteria, the HPr protein is the master regulator of carbon
metabolism. HPr is a key component of the phosphoenolpyruvate (PEP): sugar …

Post-translational modifications in Pseudomonas aeruginosa revolutionized by proteomic analysis

T Ouidir, T Jouenne, J Hardouin - Biochimie, 2016 - Elsevier
Pseudomonas aeruginosa is an opportunistic pathogen that causes severe infections in
vulnerable individuals. It is known that post-translational modifications (PTMs) play a key …

Global quantification of phosphoproteins combining metabolic labeling and gel‐based proteomics in B. pumilus

C Hentschker, C Dewald, A Otto, K Büttner… - …, 2018 - Wiley Online Library
Differential proteomics targeting the protein abundance is commonly used to follow changes
in biological systems. Differences in localization and degree of post‐translational …

Potential of liquid-isoelectric-focusing protein fractionation to improve phosphoprotein characterization of Pseudomonas aeruginosa PA14

T Ouidir, F Jarnier, P Cosette, T Jouenne… - Analytical and …, 2014 - Springer
Protein phosphorylation on serine, threonine, and tyrosine is known to be involved in a wide
variety of cellular processes and signal transduction in bacteria. Bacterial-proteome analysis …

The analysis of Neisseria meningitidis proteomes: Reference maps and their applications

G Bernardini, D Braconi, A Santucci - Proteomics, 2007 - Wiley Online Library
Neisseria meningitidis is an encapsulated Gram‐negative bacterium responsible for
significant morbidity and mortality worldwide. The availability of meningococcal genome …

Comparative analysis of gene expression on mRNA and protein level during development of Streptomyces cultures by using singular value decomposition

J Vohradsky, P Branny, CJ Thompson - Proteomics, 2007 - Wiley Online Library
This paper describes a comparative systems level analysis of the developmental proteome
and transcriptome in the model antibiotic‐producing eubacterium Streptomyces coelicolor …