[HTML][HTML] CRISPR in cancer biology and therapy
Over the past decade, CRISPR has become as much a verb as it is an acronym,
transforming biomedical research and providing entirely new approaches for dissecting all …
transforming biomedical research and providing entirely new approaches for dissecting all …
[HTML][HTML] Enrichment strategies to enhance genome editing
NS Mikkelsen, RO Bak - Journal of Biomedical Science, 2023 - Springer
Genome editing technologies hold great promise for numerous applications including the
understanding of cellular and disease mechanisms and the development of gene and …
understanding of cellular and disease mechanisms and the development of gene and …
Base editing sensor libraries for high-throughput engineering and functional analysis of cancer-associated single nucleotide variants
FJ Sánchez-Rivera, BJ Diaz, ER Kastenhuber… - Nature …, 2022 - nature.com
Base editing can be applied to characterize single nucleotide variants of unknown function,
yet defining effective combinations of single guide RNAs (sgRNAs) and base editors …
yet defining effective combinations of single guide RNAs (sgRNAs) and base editors …
Generation of precision preclinical cancer models using regulated in vivo base editing
Although single-nucleotide variants (SNVs) make up the majority of cancer-associated
genetic changes and have been comprehensively catalogued, little is known about their …
genetic changes and have been comprehensively catalogued, little is known about their …
Obesity promotes breast epithelium DNA damage in women carrying a germline mutation in BRCA1 or BRCA2
Obesity, defined as a body mass index (BMI)≥ 30, is an established risk factor for breast
cancer among women in the general population after menopause. Whether elevated BMI is …
cancer among women in the general population after menopause. Whether elevated BMI is …
Rescue of hearing by adenine base editing in a humanized mouse model of Usher syndrome type 1F
Usher syndrome type 1F (USH1F), characterized by congenital lack of hearing and balance
and progressive loss of vision, is caused by mutations in the PCDH15 gene. In the …
and progressive loss of vision, is caused by mutations in the PCDH15 gene. In the …
PEAR, a flexible fluorescent reporter for the identification and enrichment of successfully prime edited cells
Prime editing is a recently developed CRISPR/Cas9 based gene engineering tool that
allows the introduction of short insertions, deletions, and substitutions into the genome …
allows the introduction of short insertions, deletions, and substitutions into the genome …
[HTML][HTML] Mutation-specific reporter for optimization and enrichment of prime editing
IF Schene, IP Joore, JHL Baijens, R Stevelink… - Nature …, 2022 - nature.com
Prime editing is a versatile genome-editing technique that shows great promise for the
generation and repair of patient mutations. However, some genomic sites are difficult to edit …
generation and repair of patient mutations. However, some genomic sites are difficult to edit …
[HTML][HTML] A luciferase reporter mouse model to optimize in vivo gene editing validated by lipid nanoparticle delivery of adenine base editors
SY Yu, T Carlaw, T Thomson, A Birkenshaw, G Basha… - Molecular Therapy, 2023 - cell.com
The rapid development of CRISPR genome editing technology has provided the potential to
treat genetic diseases effectively and precisely. However, efficient and safe delivery of …
treat genetic diseases effectively and precisely. However, efficient and safe delivery of …
[HTML][HTML] In Vivo Rapid Investigation of CRISPR-Based Base Editing Components in Escherichia coli (IRI-CCE): A Platform for Evaluating Base Editing Tools and Their …
Rapid assessment of clustered regularly interspaced short palindromic repeats/CRISPR-
associated protein (CRISPR/Cas)-based genome editing (GE) tools and their components is …
associated protein (CRISPR/Cas)-based genome editing (GE) tools and their components is …