Droplet-based microfluidics in biomedical applications
Droplet-based microfluidic systems have been employed to manipulate discrete fluid
volumes with immiscible phases. Creating the fluid droplets at microscale has led to a …
volumes with immiscible phases. Creating the fluid droplets at microscale has led to a …
Development and evaluation of a rapid CRISPR-based diagnostic for COVID-19
T Hou, W Zeng, M Yang, W Chen, L Ren, J Ai… - PLoS …, 2020 - journals.plos.org
The recent outbreak of human infections caused by SARS-CoV-2, the third zoonotic
coronavirus has raised great public health concern globally. Rapid and accurate diagnosis …
coronavirus has raised great public health concern globally. Rapid and accurate diagnosis …
[HTML][HTML] An improvement of the 2ˆ (–delta delta CT) method for quantitative real-time polymerase chain reaction data analysis
Background The 2-ΔΔ CT method has been extensively used as a relative quantification
strategy for quantitative real-time polymerase chain reaction (qPCR) data analysis. This …
strategy for quantitative real-time polymerase chain reaction (qPCR) data analysis. This …
Rapid and fully microfluidic Ebola virus detection with CRISPR-Cas13a
Highly infectious illness caused by pathogens is endemic especially in developing nations
where there is limited laboratory infrastructure and trained personnel. Rapid point-of-care …
where there is limited laboratory infrastructure and trained personnel. Rapid point-of-care …
Detection of contaminants in water supply: A review on state-of-the-art monitoring technologies and their applications
Water monitoring technologies are widely used for contaminants detection in wide variety of
water ecology applications such as water treatment plant and water distribution system. A …
water ecology applications such as water treatment plant and water distribution system. A …
[HTML][HTML] Variability in RT-qPCR assay parameters indicates unreliable SARS-CoV-2 RNA quantification for wastewater surveillance
Due to the coronavirus disease 2019 (COVID-19) pandemic, wastewater surveillance has
become an important tool for monitoring the spread of severe acute respiratory syndrome …
become an important tool for monitoring the spread of severe acute respiratory syndrome …
Correction of RT–qPCR data for genomic DNA-derived signals with ValidPrime
H Laurell, JS Iacovoni, A Abot, D Svec… - Nucleic acids …, 2012 - academic.oup.com
Genomic DNA (gDNA) contamination is an inherent problem during RNA purification that
can lead to non-specific amplification and aberrant results in reverse transcription …
can lead to non-specific amplification and aberrant results in reverse transcription …
Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data
JM Ruijter, C Ramakers, WMH Hoogaars… - Nucleic acids …, 2009 - academic.oup.com
Despite the central role of quantitative PCR (qPCR) in the quantification of mRNA
transcripts, most analyses of qPCR data are still delegated to the software that comes with …
transcripts, most analyses of qPCR data are still delegated to the software that comes with …
In situ amplification‐based imaging of RNA in living cells
Owing to its important physiological functions, especially as molecular biomarkers of
diseases, RNA is an important focus of biomedicine and biochemical sensing. Signal …
diseases, RNA is an important focus of biomedicine and biochemical sensing. Signal …