Detecting the coronavirus (COVID-19)

P Pokhrel, C Hu, H Mao - ACS sensors, 2020 - ACS Publications
The COVID-19 pandemic has created huge damage to society and brought panic around
the world. Such panic can be ascribed to the seemingly deceptive features of COVID-19 …

Droplet-based microfluidics in biomedical applications

L Amirifar, M Besanjideh, R Nasiri, A Shamloo… - …, 2022 - iopscience.iop.org
Droplet-based microfluidic systems have been employed to manipulate discrete fluid
volumes with immiscible phases. Creating the fluid droplets at microscale has led to a …

Development and evaluation of a rapid CRISPR-based diagnostic for COVID-19

T Hou, W Zeng, M Yang, W Chen, L Ren, J Ai… - PLoS …, 2020 - journals.plos.org
The recent outbreak of human infections caused by SARS-CoV-2, the third zoonotic
coronavirus has raised great public health concern globally. Rapid and accurate diagnosis …

[HTML][HTML] An improvement of the 2ˆ (–delta delta CT) method for quantitative real-time polymerase chain reaction data analysis

X Rao, X Huang, Z Zhou, X Lin - Biostatistics, bioinformatics and …, 2013 - ncbi.nlm.nih.gov
Background The 2-ΔΔ CT method has been extensively used as a relative quantification
strategy for quantitative real-time polymerase chain reaction (qPCR) data analysis. This …

Rapid and fully microfluidic Ebola virus detection with CRISPR-Cas13a

P Qin, M Park, KJ Alfson, M Tamhankar, R Carrion… - ACS …, 2019 - ACS Publications
Highly infectious illness caused by pathogens is endemic especially in developing nations
where there is limited laboratory infrastructure and trained personnel. Rapid point-of-care …

Detection of contaminants in water supply: A review on state-of-the-art monitoring technologies and their applications

SN Zulkifli, HA Rahim, WJ Lau - Sensors and Actuators B: Chemical, 2018 - Elsevier
Water monitoring technologies are widely used for contaminants detection in wide variety of
water ecology applications such as water treatment plant and water distribution system. A …

[HTML][HTML] Variability in RT-qPCR assay parameters indicates unreliable SARS-CoV-2 RNA quantification for wastewater surveillance

A Bivins, D Kaya, K Bibby, SL Simpson, SA Bustin… - Water Research, 2021 - Elsevier
Due to the coronavirus disease 2019 (COVID-19) pandemic, wastewater surveillance has
become an important tool for monitoring the spread of severe acute respiratory syndrome …

Correction of RT–qPCR data for genomic DNA-derived signals with ValidPrime

H Laurell, JS Iacovoni, A Abot, D Svec… - Nucleic acids …, 2012 - academic.oup.com
Genomic DNA (gDNA) contamination is an inherent problem during RNA purification that
can lead to non-specific amplification and aberrant results in reverse transcription …

Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data

JM Ruijter, C Ramakers, WMH Hoogaars… - Nucleic acids …, 2009 - academic.oup.com
Despite the central role of quantitative PCR (qPCR) in the quantification of mRNA
transcripts, most analyses of qPCR data are still delegated to the software that comes with …

In situ amplification‐based imaging of RNA in living cells

Z Qing, J Xu, J Hu, J Zheng, L He, Z Zou… - Angewandte …, 2019 - Wiley Online Library
Owing to its important physiological functions, especially as molecular biomarkers of
diseases, RNA is an important focus of biomedicine and biochemical sensing. Signal …