AlphaFold has burst into our lives. A powerful algorithm that underscores the strength of
biological sequence data and artificial intelligence (AI). AlphaFold has appended projects …

Highlights•AlphaFold models have rigid protein structures.•Advanced deep algorithms fed
relevant data can accurately predict structures.•Algorithmic advances can harness …

Allosteric communication between distant sites in proteins is central to biological regulation
but still poorly characterized, limiting understanding, engineering and drug development …

Allosteric regulation, induced by perturbations at an allosteric site topographically distinct
from the orthosteric site, is one of the most direct and efficient ways to fine-tune …

Allostery is a universal, biological phenomenon in which orthosteric sites are fine‐tuned by
topologically distal allosteric sites triggered by perturbations, such as ligand binding, residue …

Insulin‐degrading enzyme (IDE) function goes far beyond its known proteolytic role as a
regulator of insulin levels. IDE has a wide substrate promiscuity, degrading several proteins …

Isothermal titration calorimetry (ITC) involves accurately measuring the heat that is released
or absorbed in real time when one solution is titrated into another. This technique is usually …

Here, we discuss the principles of allosteric activating mutations, propagation downstream of
the signals that they prompt, and allosteric drugs, with examples from the Ras signaling …

Traditionally, drugs are monovalent, targeting only one site on the protein surface. This
includes orthosteric and allosteric drugs, which bind the protein at orthosteric and allosteric …

The recent discovery of activator compounds binding to an allosteric site on the NAD+-
dependent protein lysine deacetylase, sirtuin 6 (SIRT6) has attracted interest and presents a …