Applied biotransformations in green solvents

MJ Hernáiz, AR Alcántara, JI García… - … –A European Journal, 2010 - Wiley Online Library
The definite interest in implementing sustainable industrial technologies has impelled the
use of biocatalysts (enzymes or cells), leading to high chemo‐, regio‐and stereoselectivities …

Highly efficient synthesis of chiral alcohols with a novel NADH-dependent reductase from Streptomyces coelicolor

LJ Wang, CX Li, Y Ni, J Zhang, X Liu, JH Xu - Bioresource technology, 2011 - Elsevier
An NADH-dependent reductase (ScCR) from Streptomyces coelicolor was discovered by
genome mining for carbonyl reductases. ScCR was overexpressed in Escherichia coli BL21 …

[HTML][HTML] Genome-scale metabolic reconstruction and in silico analysis of methylotrophic yeast Pichia pastoris for strain improvement

BKS Chung, S Selvarasu, A Camattari, J Ryu… - Microbial cell …, 2010 - Springer
Background Pichia pastoris has been recognized as an effective host for recombinant
protein production. A number of studies have been reported for improving this expression …

Asymmetric reduction of ketones with recombinant E. coli whole cells in neat substrates

A Jakoblinnert, R Mladenov, A Paul, F Sibilla… - Chemical …, 2011 - pubs.rsc.org
Asymmetric reduction of ketones with recombinant E. coli whole cells in neat substrates -
Chemical Communications (RSC Publishing) DOI:10.1039/C1CC14097C Royal Society of …

[HTML][HTML] Deciphering chloramphenicol biotransformation mechanisms and microbial interactions via integrated multi-omics and cultivation-dependent approaches

J Zhang, X Li, U Klümper, H Lei, TU Berendonk, F Guo… - Microbiome, 2022 - Springer
Background As a widely used broad-spectrum antibiotic, chloramphenicol is prone to be
released into environments, thus resulting in the disturbance of ecosystem stability as well …

Unveiling the hidden performance of whole cells in the asymmetric bioreduction of aryl‐containing ketones in aqueous deep eutectic solvents

P Vitale, VM Abbinante, FM Perna… - Advanced Synthesis …, 2017 - Wiley Online Library
In this contribution, we report the first successful baker's yeast reduction of arylpropanones
using deep eutectic solvents (DESs) as biodegradable and non‐hazardous co‐solvents …

Enantioselective synthesis of pure (R, R)-2, 3-butanediol in Escherichia coli with stereospecific secondary alcohol dehydrogenases

Y Yan, CC Lee, JC Liao - Organic & biomolecular chemistry, 2009 - pubs.rsc.org
We characterized the activity and stereospecificity of four secondary alcohol
dehydrogenases (sADHs) towards acetoin reduction and constructed synthetic pathways in …

Chiral pesticides

BS Sekhon - Journal of Pesticide Science, 2009 - jstage.jst.go.jp
The enantiomers of chiral pesticides are often metabolized at different rates. In agriculture,
the preferred chiral form of a pesticide is more effective at lower application rates or more …

[HTML][HTML] Rules for biocatalyst and reaction engineering to implement effective, NAD (P) H-dependent, whole cell bioreductions

R Kratzer, JM Woodley, B Nidetzky - Biotechnology advances, 2015 - Elsevier
Access to chiral alcohols of high optical purity is today frequently provided by the enzymatic
reduction of precursor ketones. However, bioreductions are complicated by the need for …

One-Way Biohydrogen Transfer for Oxidation of sec-Alcohols

I Lavandera, A Kern, V Resch, B Ferreira-Silva… - Organic …, 2008 - ACS Publications
Quasi-irreversible oxidation of sec-alcohols was achieved via biocatalytic hydrogen transfer
reactions using alcohol dehydrogenases employing selected ketones as hydrogen …