The RNA synthesis of porcine epidemic diarrhea virus (PEDV) is a sophisticated process
performed by a multilingual viral replication complex, together with cellular factors. A key …

Recombinant expression of human membrane proteins in large quantities remains a major
challenge. Expression host is an important variable to screen for high-level production of …

Intrinsically disordered proteins (IDPs) are an emerging concept. IDPs have high flexibility in
their polypeptide chains, lacking a stable 3-dimensional structure. Because of the difficulty in …

AU-rich elements in 5'untranslated region (UTR) are known to increase translation efficiency
by recruiting S1 protein that facilitates the assembly of ribosomes. However, AU-rich …

Fusokines are chimeric proteins generated by the physical coupling of cytokines in a single
polypeptide, resulting in proteins with highly pleiotropic activity and the potential to treat …

Many proteins contain intrinsically disordered regions that are highly solvent-exposed and
susceptible to post-translational modifications. Studying these protein segments is critical to …

Background and purpose: Clostridium perfringens is an anaerobic, spore-forming, and
pathogenic bacterium that causes intestinal diseases in humans and animals. In these …

The formation of inclusion bodies (IBs) without enzyme activity in bacterial research is
generally undesirable. Researchers have attempted to recovery the enzyme activities of IBs …

The purification of membrane proteins can be challenging due to their low solubility in
conventional detergents and/or chaotropic solutions. The introduction of fusion systems that …

Introducing one or more intramolecular thioether bridges in a peptide provides a promising
approach to create more stable molecules with improved pharmacodynamic properties and …