Development of clone with novel TrpE fusion tag in E. coli for overexpression of trypsin in a bench-scale bioreactor
SN Nanjundaiah, J Ma, S Sukumaran… - Preparative …, 2021 - Taylor & Francis
Trypsin is a key enzyme under the serine proteases that is found in the pancreas which
plays a key role in protein digestion. It cleaves peptide chains mainly at the carboxyl side of …
plays a key role in protein digestion. It cleaves peptide chains mainly at the carboxyl side of …
Single step purification of recombinant proteins using the metal ion-inducible autocleavage (MIIA) domain as linker for tag removal
S Ibe, J Schirrmeister, S Zehner - Journal of Biotechnology, 2015 - Elsevier
For fast and easy purification, proteins are typically fused with an affinity tag, which often
needs to be removed after purification. Here, we present a method for the removal of the …
needs to be removed after purification. Here, we present a method for the removal of the …
Facile production of tagless membrane scaffold protein for nanodiscs
JA Julien, SG Mutchek, MG Fernandez, KJ Glover - Analytical biochemistry, 2022 - Elsevier
The initial step in the preparation of nanodiscs is to express and purify the membrane
scaffold protein (MSP) to homogeneity. Current methods used for the isolation and …
scaffold protein (MSP) to homogeneity. Current methods used for the isolation and …
[PDF][PDF] Designing and overproducing a tandem epitope of gp350/220 that shows a potential to become an EBV vaccine
NVM Anyndita, N Dluha, M Rifa'i, K Himmah… - Heliyon, 2018 - cell.com
Abstract Background Epstein-Barr virus (EBV) can cause cancer in people from around the
world. There is no EBV vaccine available for use on a global scale. However, emerging …
world. There is no EBV vaccine available for use on a global scale. However, emerging …
Determinación de estructuras cristalográficas mediante el uso de proteínas de fusión
MCC Echavarría - 2024 - riaa.uaem.mx
Las proteínas de fusión constituyen una herramienta biotecnológica, que suelen ser usadas
como biosensores, para mejoramiento enzimático, fármacos, purificación de proteínas entre …
como biosensores, para mejoramiento enzimático, fármacos, purificación de proteínas entre …
[图书][B] Protease-Catalyzed Synthesis of N-Acryloyl-Oligopeptides and Histidine-Containing Oligopeptides
C Edson - 2022 - search.proquest.com
Synthetic peptides, peptides built via chemical methodology or through recombinant
technology, have been designed for use in therapeutics. Current chemical methods to create …
technology, have been designed for use in therapeutics. Current chemical methods to create …
[图书][B] Production and Purification of Basic Fibroblast Growth Factor Fused to two Collagen Binding Domains expressed in E. coli BL21 using Flask and fed-Batch
H Aljewari - 2019 - search.proquest.com
Delivering effective and non-toxic doses of bioactive materials that can aid in activating
tissue regeneration to wounded tissue has proven to be an enormous challenge. This study …
tissue regeneration to wounded tissue has proven to be an enormous challenge. This study …
Optimalisation of expression conditions for production of round-leaf sundew chitinase (Drosera rotundifolia L.) in three E. coli expression strains
M Rajninec, J Libantová, M Jopčík - Journal of Central European …, 2016 - hrcak.srce.hr
Round-leaf sundew (Drosera rotundifolia L.), family Droseraceae, genus Drosera, is one of a
few plant species with a strong antifungal potential. Chitinases of carnivorous plants play an …
few plant species with a strong antifungal potential. Chitinases of carnivorous plants play an …
[HTML][HTML] A Simple Method for High-Level Expression of HTLV-1 gp46-I (162-214) Peptide and Its Application for Screening
S Behdad, S Dolatabadi, J Saeidi - Zahedan Journal of Research in …, 2018 - zjrms.com
Background: Gp46-I (162-214) is a linear peptide derived from human T-lymphotropic virus
Type 1 (HTLV-1) gp46-I envelope protein. This protein is commonly used to detect HTLV-1 …
Type 1 (HTLV-1) gp46-I envelope protein. This protein is commonly used to detect HTLV-1 …
重组人βNGF 在大肠杆菌中的可溶表达, 纯化及活性鉴定
白羊, 章永垒, 邹有土, 黄奋飞, 陈胜亮, 阮卡… - 生物技术 …, 2016 - biotech.aiijournal.com
旨在大肠杆菌中可溶表达重组人神经生长因子(Recombinant human β nerve growth factor,
rhβNGF), 并对表达产物进行分离纯化和生物学活性鉴定. 成功扩增hNGF β 亚基基因 …
rhβNGF), 并对表达产物进行分离纯化和生物学活性鉴定. 成功扩增hNGF β 亚基基因 …