Evidence of Multiple Regulatory Functions for the PtsN (IIANtr) Protein of Pseudomonas putida

I Cases, JA Lopez, JP Albar… - Journal of Bacteriology, 2001 - Am Soc Microbiol
The ptsN gene of Pseudomonas putida encodes IIANtr, a protein of the phosphoenol
pyruvate: sugar phosphotransferase (PTS) system which is required for the C source …

Inactivation of Pseudomonas putida KT2440 pyruvate dehydrogenase relieves catabolite repression and improves the usefulness of this strain for degrading aromatic …

R Moreno, L Yuste, G Morales… - Microbial …, 2024 - Wiley Online Library
Pyruvate dehydrogenase (PDH) catalyses the irreversible decarboxylation of pyruvate to
acetyl‐CoA, which feeds the tricarboxylic acid cycle. We investigated how the loss of PDH …

Genetic Evidence that Catabolites of the Entner-Doudoroff Pathway Signal C Source Repression of the σ54Pu Promoter of Pseudomonas putida

F Velázquez, I Di Bartolo, V De Lorenzo - Journal of bacteriology, 2004 - Am Soc Microbiol
Glucose and other C sources exert an atypical form of catabolic repression on the σ54-
dependent promoter Pu, which drives transcription of an operon for m-xylene degradation …

Linking genes to microbial growth kinetics—An integrated biochemical systems engineering approach

M Koutinas, A Kiparissides, R Silva-Rocha… - Metabolic …, 2011 - Elsevier
The majority of models describing the kinetic properties of a microorganism for a given
substrate are unstructured and empirical. They are formulated in this manner so that the …

Catabolite repression and physiological control

F Rojo, M Alejandro Dinamarca - Virulence and Gene Regulation, 2004 - Springer
When confronted with a mixture of potential carbon sources at sufficiently high
concentrations, many bacterial species often assimilate the different compounds in an …

Novel physiological modulation of the Pu promoter of TOL plasmid: negative regulatory role of the TurA protein of Pseudomonas putida in the response to suboptimal …

E Rescalli, S Saini, C Bartocci, L Rychlewski… - Journal of Biological …, 2004 - ASBMB
From crude protein extracts of Pseudomonas putida KT2440, we identified a small protein,
TurA, able to bind to DNA fragments bearing the entire Pu promoter sequence of the TOL …

Role of the ptsN Gene Product in Catabolite Repression of the Pseudomonas putida TOL Toluene Degradation Pathway in Chemostat Cultures

I Aranda-Olmedo, P Marín, JL Ramos… - Applied and …, 2006 - Am Soc Microbiol
ABSTRACT The Pseudomonas putida KT2440 TOL upper pathway is repressed under
nonlimiting conditions in cells growing in chemostat with succinate as a carbon source. We …

Cooperative amino acid changes shift the response of the σ54‐dependent regulator XylR from natural m‐xylene towards xenobiotic 2,4‐dinitrotoluene

A de Las Heras, V de Lorenzo - Molecular microbiology, 2011 - Wiley Online Library
XylR is a σ54‐dependent transcriptional factor of Pseudomonas putida that activates the Pu
promoter of the TOL plasmid upon binding its natural effector, m‐xylene. The search for …

The acetoin assimilation pathway of Pseudomonas putida KT2440 is regulated by overlapping global regulatory elements that respond to nutritional cues

R Moreno, L Yuste, F Rojo - Environmental microbiology, 2023 - Wiley Online Library
Many microorganisms produce and excrete acetoin (3‐hydroxy‐2‐butanone) when growing
in environments that contain glucose or other fermentable carbon sources. This excreted …

Recruitment of σ54-RNA polymerase to the Pu promoter of Pseudomonas putida through integration host factor-mediated positioning switch of α subunit carboxyl …

R Macchi, L Montesissa, K Murakami… - Journal of Biological …, 2003 - ASBMB
The interactions between the σ 54-containing RNA polymerase (σ 54-RNAP) and the region
of the Pseudomonas putida Pu promoter spanning from the enhancer to the binding site for …