Genome editing continues to revolutionize biological research. Due to its simplicity and
flexibility, CRISPR/Cas-based editing has become the preferred technology in most systems …

Adenine base editors (ABEs) catalyze A-to-G transitions showing broad applications, but
their bystander mutations and off-target editing effects raise safety concerns. Through …

Our ability to edit genomes lags behind our capacity to sequence them, but the growing
understanding of CRISPR biology and its application to genome, epigenome and …

Background Testing an ever-increasing number of CRISPR components is challenging
when developing new genome engineering tools. Plant biotechnology has few high …

Adenine base editors (ABEs) are valuable, precise genome editing tools in plants. In recent
years, the highly promising ADENINE BASE EDITOR8e (ABE8e) was reported for efficient A …

Base editors (BEs) empower the efficient installation of beneficial or corrective point
mutations in crop and human genomes. However, conventional BEs can induce …

CRISPR‐based directed evolution is an effective breeding biotechnology to improve
agronomic traits in plants. However, its gene diversification is still limited using individual …

Transcriptional regulation is crucial to control of gene expression. Both spatio-temporal
expression patterns and expression levels of genes are determined by the interaction …

Scientists have developed many approaches based on PCR or next-generation sequencing
to localize and characterize integrated T-DNAs in transgenic plants generated by …

Background CRISPR/Cas-derived base editor enables precise editing of target sites and
has been widely used for basic research and crop genetic improvement. However, the …