In-cell structural biology by NMR: the benefits of the atomic scale
FX Theillet - Chemical reviews, 2022 - ACS Publications
In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …
acids in their native, cellular environment. This emerging field holds great promise and is …
Synthetic biology—the synthesis of biology
S Ausländer, D Ausländer… - Angewandte Chemie …, 2017 - Wiley Online Library
Synthetic biology concerns the engineering of man‐made living biomachines from
standardized components that can perform predefined functions in a (self‐) controlled …
standardized components that can perform predefined functions in a (self‐) controlled …
Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts
The mammalian microbiome has many important roles in health and disease,, and genetic
engineering is enabling the development of microbial therapeutics and diagnostics,,,,. A key …
engineering is enabling the development of microbial therapeutics and diagnostics,,,,. A key …
Burden-driven feedback control of gene expression
Cells use feedback regulation to ensure robust growth despite fluctuating demands for
resources and differing environmental conditions. However, the expression of foreign …
resources and differing environmental conditions. However, the expression of foreign …
Genomically mined acoustic reporter genes for real-time in vivo monitoring of tumors and tumor-homing bacteria
Ultrasound allows imaging at a much greater depth than optical methods, but existing
genetically encoded acoustic reporters for in vivo cellular imaging have been limited by poor …
genetically encoded acoustic reporters for in vivo cellular imaging have been limited by poor …
Characterization and mitigation of gene expression burden in mammalian cells
Despite recent advances in circuit engineering, the design of genetic networks in
mammalian cells is still painstakingly slow and fraught with inexplicable failures. Here, we …
mammalian cells is still painstakingly slow and fraught with inexplicable failures. Here, we …
Diverse genetic error modes constrain large-scale bio-based production
A transition toward sustainable bio-based chemical production is important for green growth.
However, productivity and yield frequently decrease as large-scale microbial fermentation …
However, productivity and yield frequently decrease as large-scale microbial fermentation …
Stress-driven dynamic regulation of multiple tolerance genes improves robustness and productive capacity of Saccharomyces cerevisiae in industrial lignocellulose …
L Qin, S Dong, J Yu, X Ning, K Xu, SJ Zhang, L Xu… - Metabolic …, 2020 - Elsevier
Yeast productivity in lignocellulosic ethanol fermentation is clearly impeded by stress.
Enhancing the robustness of xylose-fermenting yeast is important for improving …
Enhancing the robustness of xylose-fermenting yeast is important for improving …
Designing biological circuits: synthetic biology within the operon model and beyond
MA English, RV Gayet, JJ Collins - Annual Review of …, 2021 - annualreviews.org
In 1961, Jacob and Monod proposed the operon model of gene regulation. At the model's
core was the modular assembly of regulators, operators, and structural genes. To illustrate …
core was the modular assembly of regulators, operators, and structural genes. To illustrate …
Synthetic addiction extends the productive life time of engineered Escherichia coli populations
P Rugbjerg, K Sarup-Lytzen, M Nagy… - Proceedings of the …, 2018 - National Acad Sciences
Bio-production of chemicals is an important driver of the societal transition toward
sustainability. However, fermentations with heavily engineered production organisms can be …
sustainability. However, fermentations with heavily engineered production organisms can be …