25th ANNIVERSARY OF CLONING BY SOMATIC-CELL NUCLEAR TRANSFER: Current applications of SCNT in advanced breeding and genome editing in livestock

C Galli, G Lazzari - Reproduction, 2021 - rep.bioscientifica.com
SCNT (somatic cell nuclear transfer) has complemented the toolbox of ARTs offering yet
another technique to reproduce animals in an unprecedented way. Despite remarkable …

Cryopreservation of porcine embryos: Recent updates and progress

D Xingzhu, Z Qingrui, C Keren, L Yuxi… - Biopreservation and …, 2021 - liebertpub.com
Cryopreservation of embryos is important for long-distance embryo transfer and
conservation of genetic resources. Porcine research is important for animal husbandry and …

Generation of nonmosaic, two-pore channel 2 biallelic knockout pigs in one generation by CRISPR-Cas9 microinjection before oocyte insemination

S Navarro-Serna, A Hachem, A Canha-Gouveia… - The CRISPR …, 2021 - liebertpub.com
Studies of knockout (KO) mice with defects in the endolysosomal two-pore channels (TPCs)
have shown TPCs to be involved in pathophysiological processes, including heart and …

The open cryotop system is effective for the simultaneous vitrification of a large number of porcine embryos at different developmental stages

A Gonzalez-Plaza, JM Cambra, I Parrilla… - Frontiers in Veterinary …, 2022 - frontiersin.org
The Superfine Open Pulled Straw (SOPS) system is the most commonly used method for
vitrification of pig embryos. However, this system only allows the vitrification of four to seven …

Generation of gene edited pigs

S Navarro-Serna, C Piñeiro-Silva, R Romar… - … Agriculture Reviews 57 …, 2022 - Springer
Abstract Background: The porcine species (Sus scrofa domesticus) has had a great impact
as a source of food worldwide, but also it is a very useful animal for biomedical applications …

[HTML][HTML] Cryotop vitrification of large batches of pig embryos simultaneously provides excellent postwarming survival rates and minimal interference with gene …

A Gonzalez-Plaza, JM Cambra, M Garcia-Canovas… - Theriogenology, 2023 - Elsevier
The most commonly used technique to vitrify pig embryos is the super open pulled straw
(SOPS), where a maximum of 6 embryos can be vitrified simultaneously per device without …

Unveiling how vitrification affects the porcine blastocyst: clues from a transcriptomic study

C Almiñana, F Dubuisson, S Bauersachs… - Journal of Animal …, 2022 - Springer
Background Currently, there is a high demand for efficient pig embryo cryopreservation
procedures in the porcine industry as well as for genetic diversity preservation and research …

[HTML][HTML] Boar seminal plasma: current insights on its potential role for assisted reproductive technologies in swine

I Parrilla, EA Martinez, MA Gil, C Cuello, J Roca… - Animal …, 2020 - SciELO Brasil
Seminal plasma (SP) supports not only sperm function but also the ability of spermatozoa to
withstand biotechnological procedures as artificial insemination, freezing or sex sorting …

[HTML][HTML] A decreased expression of interferon stimulated genes in peri-implantation endometrium of embryo transfer recipient sows could contribute to embryo death

CA Martinez, M Alvarez-Rodriguez… - animal, 2022 - Elsevier
Pig pregnancy succeeds thanks to a well-coordinated system ruling both maternal immune
activation and embryonic antigen tolerance. In physiological pregnancies, the maternal …

The use of a brief synchronization treatment after weaning, combined with superovulation, has moderate effects on the gene expression of surviving pig blastocysts

H Gonzalez-Ramiro, MA Gil, C Cuello, JM Cambra… - Animals, 2023 - mdpi.com
Simple Summary The use of combined estrus synchronization/superovulation (SS)
treatments alters ovarian and endometrial gene expression patterns, impairing follicle and …