Light-sheet fluorescence microscopy has transformed our ability to visualize and
quantitatively measure biological processes rapidly and over long time periods. In this …

Oblique plane microscopy (OPM) directly captures object information in a plane tilted from
the focal plane of the objective lens without the need for slow z-stack acquisition. This …

Rapid, high-resolution volumetric imaging without moving heavy objectives or disturbing
delicate samples remains challenging. Pupil-matched remote focusing offers a promising …

Projection imaging accelerates volumetric interrogation in fluorescence microscopy, but for
multi-cellular samples, the resulting images may lack contrast, as many structures and haze …

Quantification of microglial activation through morphometric analysis has long been a staple
of the neuroimmunologist's toolkit. Microglial morphological phenomics can be conducted …

Fundamental properties of light unavoidably impose features on images collected using
fluorescence microscopes. Accounting for these features is often critical in quantitatively …

Super-resolution imaging has rapidly emerged as an optical microscopy technique, offering
advantages of high optical resolution over the past two decades; achieving improved …

Heterogeneity investigation at the single-cell level reveals morphological and phenotypic
characteristics in cell populations. In clinical research, heterogeneity has important …

The background light from out-of-focus planes hinders resolution enhancement in structured
illumination microscopy when observing volumetric samples. Here we used selective plane …

Light-sheet fluorescence microscopy is an invaluable tool for four-dimensional biological
imaging of multicellular systems due to the rapid volumetric imaging and minimal …