CRISPR-Cas9, which imparts adaptive immunity against foreign genomic invaders in certain
prokaryotes, has been repurposed for genome-engineering applications. More recently …

G protein‐coupled receptors (GPCRs) play critical roles in the regulation of human
physiology in response to a wide array of different extracellular stimuli and thus represent …

A novel dark through-bond energy transfer (DTBET) strategy is proposed and applied as the
design strategy to develop ratiometric Hg2+ sensors with high performance …

Membrane-less organelles are cellular structures which arise through the phenomenon of
phase separation. This process enables compartmentalization of specific sets of …

Fluorescence applications requiring high photostability often depend on the use of solution
additives to enhance fluorophore performance. Here we demonstrate that the direct or …

Supramolecular systems have applications in areas as diverse as materials science,
biochemistry, analytical chemistry, and nanomedicine. However, analyzing such systems …

Here, we explore the enhancement of single-molecule emission by a polymeric
nanoantenna that can harvest energy from thousands of donor dyes to a single acceptor. In …

Fluorescence resonance energy transfer (FRET) is a powerful spectroscopic method for
measuring distances in the 2–8 nm range. Often, conformational changes and molecular …

An essential skill for every researcher is to learn how to select and apply the most
appropriate methods for the questions they are trying to answer. With the extensive variety of …

Highlights•Hybrid-FRET on rails automates FRET-based integrative structural
modeling.•Automated experiment design assures highest structural quality with minimal …