Rigorous characterization of urinary extracellular vesicles (uEVs) in the low centrifugation pellet-a neglected source for uEVs

L Musante, SV Bontha, S La Salvia… - Scientific Reports, 2020 - nature.com
Urinary extracellular vesicles (uEVs) provide bio-markers for kidney and urogenital
diseases. Centrifugation is the most common method used to enrich uEVs. However, a …

Synthesis and characterization of glycerol dendrons, self-assembled monolayers on gold: a detailed study of their protein resistance

M Wyszogrodzka, R Haag - Biomacromolecules, 2009 - ACS Publications
Nonfouling surface coatings are of great interest for the development of advanced
biomaterials used in biomedical and marine applications. Therefore, a lot of effort has been …

Acute aluminum phosphide poisoning: The menace of phosphine exposure

D Yadav, R Bhattacharyya, D Banerjee - Clinica Chimica Acta, 2021 - Elsevier
Aluminum phosphide (AlP) is a popular fumigant used widely for the safe storage of food
grain. Although A1P is free from toxic residues, it releases phosphine which acts on …

[HTML][HTML] The mammalian peroxisomal membrane is permeable to both GSH and GSSG–Implications for intraperoxisomal redox homeostasis

MJ Ferreira, TA Rodrigues, AG Pedrosa, L Gales… - Redox Biology, 2023 - Elsevier
Despite the large amounts of H 2 O 2 generated in mammalian peroxisomes, cysteine
residues of intraperoxisomal proteins are maintained in a reduced state. The biochemistry …

Single-molecule force spectroscopy measurements of bond elongation during a bimolecular reaction

SR Koti Ainavarapu, AP Wiita, L Dougan… - Journal of the …, 2008 - ACS Publications
It is experimentally challenging to directly obtain structural information of the transition state
(TS), the high-energy bottleneck en route from reactants to products, for solution-phase …

A class of disulfide compounds suppresses ferroptosis by stabilizing GPX4

JP Liu, SY Cen, Z Xue, TX Wang, Y Gao… - ACS Chemical …, 2022 - ACS Publications
Ferroptosis is a nonapoptotic form of cell death characterized by iron-dependent lipid
peroxidation and has been implicated in multiple pathological conditions. Glutathione …

Optimization of feasibility stage for hydrogen/deuterium exchange mass spectrometry

Y Hamuro, SJ Coales - Journal of the American Society for Mass …, 2018 - ACS Publications
The practice of HDX-MS remains somewhat difficult, not only for newcomers but also for
veterans, despite its increasing popularity. While a typical HDX-MS project starts with a …

Expanding the Genetic Code of Xenopus laevis Embryos

W Brown, LA Davidson, A Deiters - ACS Chemical Biology, 2024 - ACS Publications
The incorporation of unnatural amino acids into proteins through genetic code expansion
has been successfully adapted to African claw-toed frog embryos. Six unique unnatural …

Streptococcus pyogenes hijacks host glutathione for growth and innate immune evasion

S Brouwer, MG Jespersen, CLY Ong, DMP De Oliveira… - MBio, 2022 - Am Soc Microbiol
The nasopharynx and the skin are the major oxygen-rich anatomical sites for colonization by
the human pathogen Streptococcus pyogenes (group A Streptococcus [GAS]). To establish …

Detection and quantification of protein adduction by electrophilic fatty acids: mitochondrial generation of fatty acid nitroalkene derivatives

FJ Schopfer, C Batthyany, PRS Baker… - Free Radical Biology …, 2009 - Elsevier
Nitroalkene fatty acid derivatives manifest a strong electrophilic nature, are clinically
detectable, and induce multiple transcriptionally regulated anti-inflammatory responses. At …