Biomolecular condensates have been demonstrated as a ubiquitous phenomenon in
biological systems and play a crucial role in controlling cellular functions. However, the …

In live cells, phase separation is thought to organize macromolecules into membraneless
structures known as biomolecular condensates. Here, we reconstituted transcription in …

Abstract Polycomb Group (PcG) proteins organize chromatin at multiple scales to regulate
gene expression. A conserved Sterile Alpha Motif (SAM) in the Polycomb Repressive …

Chromatin, which consists of DNA and associated proteins, contains genetic information and
is a mechanical component of the nucleus. Heterochromatic histone methylation controls …

Fluorescent proteins (FP) are frequently used for studying proteins inside cells. In advanced
fluorescence microscopy, FPs can report on additional intracellular variables. One variable …

Liquid–liquid phase separation (LLPS) emerges as a fundamental underlying mechanism
for the biological organization, especially the formation of membraneless organelles (MLOs) …

What do we know about Ki-67, apart from its usefulness as a cell proliferation biomarker in
histopathology? Discovered in 1983, the protein and its regulation of expression and …

Access to DNA is a prerequisite to the execution of essential cellular processes that include
transcription, replication, chromosomal segregation, and DNA repair. How the proteins that …

Prostate cancer is heterogeneous and patients would benefit from methods that stratify those
who are likely to respond to systemic therapy. Here, we employ single-cell assays for …

Our understanding of the physical principles organizing the genome in the nucleus is limited
by the lack of tools to directly exert and measure forces on interphase chromosomes in vivo …