Toward automated enzymatic synthesis of oligosaccharides

L Wen, G Edmunds, C Gibbons, J Zhang… - Chemical …, 2018 - ACS Publications
Oligosaccharides together with oligonucleotides and oligopeptides comprise the three major
classes of natural biopolymers. Automated systems for oligonucleotide and oligopeptide …

Human milk oligosaccharides (HMOS): structure, function, and enzyme-catalyzed synthesis

X Chen - Advances in carbohydrate chemistry and biochemistry, 2015 - Elsevier
The important roles played by human milk oligosaccharides (HMOS), the third major
component of human milk, in the health of breast-fed infants have been increasingly …

Production of human milk oligosaccharides by enzymatic and whole-cell microbial biotransformations

GA Sprenger, F Baumgärtner, C Albermann - Journal of biotechnology, 2017 - Elsevier
Human milk oligosaccharides (HMO) are almost unique constituents of breast milk and are
not found in appreciable amounts in cow milk. Due to several positive aspects of HMO for …

A new fermentation process allows large-scale production of human milk oligosaccharides by metabolically engineered bacteria

B Priem, M Gilbert, WW Wakarchuk, A Heyraud… - …, 2002 - academic.oup.com
When fed to a β-galactosidase-negative (lacZ–) Escherichia coli strain that was grown on an
alternative carbon source (such as glycerol), lactose accumulated intracellularly on …

Machine‐driven enzymatic oligosaccharide synthesis by using a peptide synthesizer

J Zhang, C Chen, MR Gadi, C Gibbons… - Angewandte …, 2018 - Wiley Online Library
For decades, researchers have endeavored to develop a general automated system to
synthesize oligosaccharides that is comparable to the preparation of oligonucleotides and …

[HTML][HTML] A single bifunctional UDP-GlcNAc/Glc 4-epimerase supports the synthesis of three cell surface glycoconjugates in Campylobacter jejuni

S Bernatchez, CM Szymanski, N Ishiyama, J Li… - Journal of Biological …, 2005 - ASBMB
The major cell-surface carbohydrates (lipooligosaccharide, capsule, and glycoprotein N-
linked heptasaccharide) of Campylobacter jejuni NCTC 11168 contain Gal and/or GalNAc …

Reassembled Biosynthetic Pathway for Large‐Scale Carbohydrate Synthesis: α‐Gal Epitope Producing “Superbug”

X Chen, Z Liu, J Zhang, W Zhang, P Kowal… - …, 2002 - Wiley Online Library
A metabolic pathway engineered Escherichia coli strain (superbug) containing one plasmid
harboring an artificial gene cluster encoding all the five enzymes in the biosynthetic pathway …

Superbeads: Immobilization in “sweet” chemistry

J Nahalka, Z Liu, X Chen… - Chemistry–A European …, 2003 - Wiley Online Library
Enzymatic oligosaccharide synthesis using recombinant glycosyltransferases is able to
overcome the difficulties associated with chemical methods. Nonetheless, sugar nucleotide …

Access to galectin-3 inhibitors from chemoenzymatic synthons

C Dussouy, S Téletchéa, A Lambert… - The Journal of …, 2020 - ACS Publications
Chemoenzymatic strategies are useful for providing both regio-and stereoselective access
to bioactive oligosaccharides. We show herein that a glycosynthase mutant of a Thermus …

P1 Trisaccharide (Galα1,4Galβ1,4GlcNAc) Synthesis by Enzyme Glycosylation Reactions Using Recombinant Escherichia coli

Z Liu, Y Lu, J Zhang, K Pardee… - Applied and …, 2003 - Am Soc Microbiol
The frequency of Escherichia coli infection has lead to concerns over pathogenic bacteria in
our food supply and a demand for therapeutics. Glycolipids on gut cells serve as receptors …