Toward automated enzymatic synthesis of oligosaccharides
Oligosaccharides together with oligonucleotides and oligopeptides comprise the three major
classes of natural biopolymers. Automated systems for oligonucleotide and oligopeptide …
classes of natural biopolymers. Automated systems for oligonucleotide and oligopeptide …
Human milk oligosaccharides (HMOS): structure, function, and enzyme-catalyzed synthesis
X Chen - Advances in carbohydrate chemistry and biochemistry, 2015 - Elsevier
The important roles played by human milk oligosaccharides (HMOS), the third major
component of human milk, in the health of breast-fed infants have been increasingly …
component of human milk, in the health of breast-fed infants have been increasingly …
Production of human milk oligosaccharides by enzymatic and whole-cell microbial biotransformations
GA Sprenger, F Baumgärtner, C Albermann - Journal of biotechnology, 2017 - Elsevier
Human milk oligosaccharides (HMO) are almost unique constituents of breast milk and are
not found in appreciable amounts in cow milk. Due to several positive aspects of HMO for …
not found in appreciable amounts in cow milk. Due to several positive aspects of HMO for …
A new fermentation process allows large-scale production of human milk oligosaccharides by metabolically engineered bacteria
B Priem, M Gilbert, WW Wakarchuk, A Heyraud… - …, 2002 - academic.oup.com
When fed to a β-galactosidase-negative (lacZ–) Escherichia coli strain that was grown on an
alternative carbon source (such as glycerol), lactose accumulated intracellularly on …
alternative carbon source (such as glycerol), lactose accumulated intracellularly on …
Machine‐driven enzymatic oligosaccharide synthesis by using a peptide synthesizer
For decades, researchers have endeavored to develop a general automated system to
synthesize oligosaccharides that is comparable to the preparation of oligonucleotides and …
synthesize oligosaccharides that is comparable to the preparation of oligonucleotides and …
[HTML][HTML] A single bifunctional UDP-GlcNAc/Glc 4-epimerase supports the synthesis of three cell surface glycoconjugates in Campylobacter jejuni
The major cell-surface carbohydrates (lipooligosaccharide, capsule, and glycoprotein N-
linked heptasaccharide) of Campylobacter jejuni NCTC 11168 contain Gal and/or GalNAc …
linked heptasaccharide) of Campylobacter jejuni NCTC 11168 contain Gal and/or GalNAc …
Reassembled Biosynthetic Pathway for Large‐Scale Carbohydrate Synthesis: α‐Gal Epitope Producing “Superbug”
X Chen, Z Liu, J Zhang, W Zhang, P Kowal… - …, 2002 - Wiley Online Library
A metabolic pathway engineered Escherichia coli strain (superbug) containing one plasmid
harboring an artificial gene cluster encoding all the five enzymes in the biosynthetic pathway …
harboring an artificial gene cluster encoding all the five enzymes in the biosynthetic pathway …
Superbeads: Immobilization in “sweet” chemistry
Enzymatic oligosaccharide synthesis using recombinant glycosyltransferases is able to
overcome the difficulties associated with chemical methods. Nonetheless, sugar nucleotide …
overcome the difficulties associated with chemical methods. Nonetheless, sugar nucleotide …
Access to galectin-3 inhibitors from chemoenzymatic synthons
C Dussouy, S Téletchéa, A Lambert… - The Journal of …, 2020 - ACS Publications
Chemoenzymatic strategies are useful for providing both regio-and stereoselective access
to bioactive oligosaccharides. We show herein that a glycosynthase mutant of a Thermus …
to bioactive oligosaccharides. We show herein that a glycosynthase mutant of a Thermus …
P1 Trisaccharide (Galα1,4Galβ1,4GlcNAc) Synthesis by Enzyme Glycosylation Reactions Using Recombinant Escherichia coli
Z Liu, Y Lu, J Zhang, K Pardee… - Applied and …, 2003 - Am Soc Microbiol
The frequency of Escherichia coli infection has lead to concerns over pathogenic bacteria in
our food supply and a demand for therapeutics. Glycolipids on gut cells serve as receptors …
our food supply and a demand for therapeutics. Glycolipids on gut cells serve as receptors …