Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …

Navigation through the bulk tumour, entry into the blood vasculature, survival in the
circulation, exit at distant sites and resumption of proliferation are all steps necessary for …

Microglia are highly motile cells that continuously monitor the brain environment and
respond to damage-associated cues. While glucose is the main energy substrate used by …

Skeletal muscle regenerates through the activation of resident stem cells. Termed satellite
cells, these normally quiescent cells are induced to proliferate by wound-derived signals …

The redox states of the NAD and NADP pyridine nucleotide pools play critical roles in
defining the activity of energy producing pathways, in driving oxidative stress and in …

Fluorescence lifetime imaging microscopy (FLIM) is used in diverse disciplines, including
biology, chemistry and biophysics, but its use has been limited by the complexity of the data …

High-throughput methods for slide-free three-dimensional (3D) pathological analyses of
whole biopsies and surgical specimens offer the promise of modernizing traditional …

NAD is a key determinant of cellular energy metabolism. In contrast, its phosphorylated form,
NADP, plays a central role in biosynthetic pathways and antioxidant defence. The reduced …

The function of a T cell depends on its subtype and activation state. Here, we show that
imaging of the autofluorescence lifetime signals of quiescent and activated T cells can be …

Fluorescence lifetime imaging (FLIM) uses the fact that the fluorescence lifetime of a
fluorophore depends on its molecular environment but not on its concentration. Molecular …