Background The engineering of functional tissues is a complex multi-stage process, the
success of which depends on the careful control of culture conditions and ultimately tissue …

Optical chemical sensors have promoted escalating interest in the determination of various
pollutants in the environment, which are creating toxicity and may cause serious health …

We propose a graphical method using the phasor representation of the fluorescence decay
to derive the absolute concentration of NADH in cells. The method requires the …

Fluorescence microscopy, in particular immunofluorescence microscopy, has been used
extensively for the assessment of kidney function and pathology for both research and …

Metabolic FLIM (fluorescence lifetime imaging) is used to image bioenergetic status in cells
and tissue. Whereas an attribution of the fluorescence lifetime of coenzymes as an indicator …

Purpose: Human pluripotent stem cell (hPSC)-derived retinal organoids are a platform for
investigating retinal development, pathophysiology, and cellular therapies. In contrast to …

We have studied physiological parameters in a living cell using fluorescence lifetime
imaging of endogenous chromophores. In this study, pH dependence of the fluorescence …

Human pluripotent stem cell (hPSC)-derived cardiomyocytes provide a promising
regenerative cell therapy for cardiovascular patients and an important model system to …

Despite extensive interest, extracellular vesicle (EV) research remains technically
challenging. One of the unexplored gaps in EV research has been the inability to …

Methods of optical spectroscopy that provide quantitative, physically or physiologically
meaningful measures of tissue properties are an attractive tool for the study, diagnosis …