Structure and folding of disulfide‐rich miniproteins: insights from molecular dynamics simulations and MM‐PBSA free energy calculations
C Combelles, J Gracy, A Heitz… - Proteins: Structure …, 2008 - Wiley Online Library
The fold of small disulfide‐rich proteins largely relies on two or more disulfide bridges that
are main components of the hydrophobic core. Because of the small size of these proteins …
are main components of the hydrophobic core. Because of the small size of these proteins …
The two cysteine-rich head domains of minicollagen from Hydra nematocysts differ in their cystine framework and overall fold despite an identical cysteine sequence …
AG Milbradt, C Boulegue, L Moroder… - Journal of molecular …, 2005 - Elsevier
Synthetic replicates of naturally occurring cysteine-rich peptides such as hormones,
neurotransmitters, growth factors, enzyme inhibitors, defensins and toxins often can be …
neurotransmitters, growth factors, enzyme inhibitors, defensins and toxins often can be …
Expression, purification, renaturation and activation of fish myostatin expressed in Escherichia coli: facilitation of refolding and activity inhibition by myostatin …
B Funkenstein, Y Rebhan - Protein Expression and Purification, 2007 - Elsevier
Myostatin (growth and differentiation factor-8) is a member of the transforming growth factor-
β superfamily, is expressed mainly in skeletal muscle and acts as a negative growth …
β superfamily, is expressed mainly in skeletal muscle and acts as a negative growth …
Heterologous protein expression in psychrophilic hosts
The vast number of candidate proteins generated from genome projects are creating
enormous opportunities for biologists. However, efficient expression of genes in …
enormous opportunities for biologists. However, efficient expression of genes in …
proNGF/NGF mixtures induce gene expression changes in PC12 cells that neither singly produces
Background Growing evidence shows that, in vivo, the precursor of Nerve Growth Factor
(NGF), proNGF, displays biological activities different from those of its mature NGF …
(NGF), proNGF, displays biological activities different from those of its mature NGF …
[HTML][HTML] Oxidative folding of nerve growth factor can be mediated by the pro-peptide of neurotrophin-3
A Hauburger, M Kliemannel, P Madsen, R Rudolph… - FEBS letters, 2007 - Elsevier
We have previously shown that the pro-peptide of human nerve growth factor (NGF)
facilitates oxidative folding of the mature part. For the analysis of functional specificities of …
facilitates oxidative folding of the mature part. For the analysis of functional specificities of …
Total chemical synthesis and oxidative folding of δ‐conotoxin PVIA containing an N‐terminal propeptide
P Buczek, O Buczek, G Bulaj - Peptide Science: Original …, 2005 - Wiley Online Library
Small disulfide‐rich peptides are translated as larger precursors typically containing an N‐
terminal prepro sequence. In this study, we investigated the role of a propeptide in the …
terminal prepro sequence. In this study, we investigated the role of a propeptide in the …
Pro-domain in precursor nerve growth factor mediates cell death
Nerve growth factor (NGF) is synthesized as a precursor, proNGF that undergoes post-
translational processing to generate the biologically active mature NGF. While the …
translational processing to generate the biologically active mature NGF. While the …
Fragments of pro‐peptide activate mature penicillin amidase of Alcaligenes faecalis
V Kasche, B Galunsky… - European journal of …, 2003 - Wiley Online Library
Penicillin amidase from Alcaligenes faecalis is a recently identified N‐terminal nucleophile
hydrolase, which possesses the highest specificity constant (kcat/Km) for the hydrolysis of …
hydrolase, which possesses the highest specificity constant (kcat/Km) for the hydrolysis of …