Pichia pastoris: A highly successful expression system for optimal synthesis of heterologous proteins

M Karbalaei, SA Rezaee… - Journal of cellular …, 2020 - Wiley Online Library
One of the most important branches of genetic engineering is the expression of recombinant
proteins using biological expression systems. Nowadays, different expression systems are …

Engineering microbial hosts for production of bacterial natural products

MM Zhang, Y Wang, EL Ang, H Zhao - Natural product reports, 2016 - pubs.rsc.org
Covering up to end 2015 Microbial fermentation provides an attractive alternative to
chemical synthesis for the production of structurally complex natural products. In most cases …

[图书][B] Biotechnology of lactic acid bacteria: novel applications. 2

F Mozzi, RR Raya, GM Vignolo, JC Love - 2015 - Wiley Online Library
Over the past 100 years, industrial biotechnology has grown into a multibillion dollar market,
which now has even begun to include large parts of chemical, material, and fuel production …

Recombinant protein production in Pichia pastoris: from transcriptionally redesigned strains to bioprocess optimization and metabolic modelling

BG Ergün, J Berrios, B Binay, P Fickers - FEMS Yeast Research, 2021 - academic.oup.com
Pichia pastoris is one of the most widely used host for the production of recombinant
proteins. Expression systems that rely mostly on promoters from genes encoding alcohol …

Microbial microdroplet culture system (MMC): An integrated platform for automated, high‐throughput microbial cultivation and adaptive evolution

X Jian, X Guo, J Wang, ZL Tan, X Xing… - Biotechnology and …, 2020 - Wiley Online Library
Conventional microbial cell cultivation techniques are typically labor intensive, low
throughput, and poorlyparallelized, rendering them inefficient. The development of …

Second generation Pichia pastoris strain and bioprocess designs

BG Ergün, K Laçın, B Çaloğlu, B Binay - Biotechnology for Biofuels and …, 2022 - Springer
Yeast was the first microorganism used by mankind for biotransformation processes that laid
the foundations of industrial biotechnology. In the last decade, Pichia pastoris has become …

Pentose metabolism and conversion to biofuels and high-value chemicals in yeasts

J Ruchala, AA Sibirny - FEMS Microbiology Reviews, 2021 - academic.oup.com
Pentose sugars are widespread in nature and two of them, D-xylose and L-arabinose
belong to the most abundant sugars being the second and third by abundance sugars in dry …

Host-Informed Expression of CRISPR Guide RNA for Genomic Engineering in Komagataella phaffii

NC Dalvie, J Leal, CA Whittaker, Y Yang… - ACS synthetic …, 2019 - ACS Publications
There is growing interest in the use of nonmodel microorganisms as hosts for
biopharmaceutical manufacturing. These hosts require genomic engineering to meet …

Pichia pastoris as a Versatile Cell Factory for the Production of Industrial Enzymes and Chemicals: Current Status and Future Perspectives

T Zhu, H Sun, M Wang, Y Li - Biotechnology Journal, 2019 - Wiley Online Library
With nearly three decades of development, Pichia pastoris (P. pastoris) has become a
powerful eukaryotic protein expression system for the expression of thousands of proteins …

Advances in Cell Engineering of the Komagataella phaffii Platform for Recombinant Protein Production

C Bustos, J Quezada, R Veas, C Altamirano… - Metabolites, 2022 - mdpi.com
Komagataella phaffii (formerly known as Pichia pastoris) has become an increasingly
important microorganism for recombinant protein production. This yeast species has gained …