[HTML][HTML] Aquatic environmental DNA: A review of the macro-organismal biomonitoring revolution

M Takahashi, M Saccò, JH Kestel, G Nester… - Science of the Total …, 2023 - Elsevier
Environmental DNA (eDNA) is the fastest growing biomonitoring tool fuelled by two key
features: time efficiency and sensitivity. Technological advancements allow rapid …

Towards the fully automated monitoring of ecological communities

M Besson, J Alison, K Bjerge, TE Gorochowski… - Ecology …, 2022 - Wiley Online Library
High‐resolution monitoring is fundamental to understand ecosystems dynamics in an era of
global change and biodiversity declines. While real‐time and automated monitoring of …

Best practices in metabarcoding of fungi: from experimental design to results

L Tedersoo, M Bahram, L Zinger, RH Nilsson… - Molecular …, 2022 - Wiley Online Library
The development of high‐throughput sequencing (HTS) technologies has greatly improved
our capacity to identify fungi and unveil their ecological roles across a variety of ecosystems …

Environmental DNA metabarcoding: A novel method for biodiversity monitoring of marine fish communities

M Miya - Annual Review of Marine Science, 2022 - annualreviews.org
Environmental DNA (eDNA) is genetic material that has been shed from macroorganisms. It
has received increased attention as an indirect marker for biodiversity monitoring. This …

Counting with DNA in metabarcoding studies: How should we convert sequence reads to dietary data?

BE Deagle, AC Thomas, JC McInnes… - Molecular …, 2019 - Wiley Online Library
Advances in DNA sequencing technology have revolutionized the field of molecular analysis
of trophic interactions, and it is now possible to recover counts of food DNA sequences from …

Environmental DNA: What's behind the term? Clarifying the terminology and recommendations for its future use in biomonitoring

J Pawlowski, L Apothéloz‐Perret‐Gentil… - Molecular …, 2020 - Wiley Online Library
The last decade brought a spectacular development of so‐called environmental (e) DNA
studies. In general,“environmental DNA” is defined as DNA isolated from environmental …

DNA metabarcoding—Need for robust experimental designs to draw sound ecological conclusions

L Zinger, A Bonin, IG Alsos, M Bálint, H Bik… - Molecular …, 2019 - Wiley Online Library
DNA metabarcoding, especially when coupled with high‐through‐put DNA sequencing, is
currently revolutionizing our capacity to assess biodiversity across a full range of taxa and …

[HTML][HTML] MiFish metabarcoding: a high-throughput approach for simultaneous detection of multiple fish species from environmental DNA and other samples

M Miya, RO Gotoh, T Sado - Fisheries Science, 2020 - Springer
We reviewed the current methodology and practices of the DNA metabarcoding approach
using a universal PCR primer pair MiFish, which co-amplifies a short fragment of fish DNA …

Perspectives and benefits of high-throughput long-read sequencing in microbial ecology

L Tedersoo, M Albertsen, S Anslan… - Applied and …, 2021 - Am Soc Microbiol
Short-read, high-throughput sequencing (HTS) methods have yielded numerous important
insights into microbial ecology and function. Yet, in many instances short-read HTS …

[HTML][HTML] The future of biotic indices in the ecogenomic era: Integrating (e) DNA metabarcoding in biological assessment of aquatic ecosystems

J Pawlowski, M Kelly-Quinn, F Altermatt… - Science of the Total …, 2018 - Elsevier
The bioassessment of aquatic ecosystems is currently based on various biotic indices that
use the occurrence and/or abundance of selected taxonomic groups to define ecological …