COVID-19: Transmission, prevention, and potential therapeutic opportunities
M Lotfi, MR Hamblin, N Rezaei - Clinica chimica acta, 2020 - Elsevier
The novel coronavirus disease (COVID-19) pandemic, caused by severe acute respiratory
syndrome coronavirus 2 (SARS-CoV-2), remains a global challenge. Despite intense …
syndrome coronavirus 2 (SARS-CoV-2), remains a global challenge. Despite intense …
Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants
The number and diversity of known CRISPR–Cas systems have substantially increased in
recent years. Here, we provide an updated evolutionary classification of CRISPR–Cas …
recent years. Here, we provide an updated evolutionary classification of CRISPR–Cas …
[HTML][HTML] Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy
The December 2019 outbreak of a novel respiratory virus, SARS-CoV-2, has become an
ongoing global pandemic due in part to the challenge of identifying symptomatic …
ongoing global pandemic due in part to the challenge of identifying symptomatic …
Programmable RNA targeting with the single-protein CRISPR effector Cas7-11
A Özcan, R Krajeski, E Ioannidi, B Lee, A Gardner… - Nature, 2021 - nature.com
CRISPR–Cas interference is mediated by Cas effector nucleases that are either components
of multisubunit complexes—in class 1 CRISPR–Cas systems—or domains of a single …
of multisubunit complexes—in class 1 CRISPR–Cas systems—or domains of a single …
Prediction of on-target and off-target activity of CRISPR–Cas13d guide RNAs using deep learning
HH Wessels, A Stirn, A Méndez-Mancilla, EJ Kim… - Nature …, 2024 - nature.com
Transcriptome engineering applications in living cells with RNA-targeting CRISPR effectors
depend on accurate prediction of on-target activity and off-target avoidance. Here we design …
depend on accurate prediction of on-target activity and off-target avoidance. Here we design …
[HTML][HTML] Programmable RNA editing with compact CRISPR–Cas13 systems from uncultivated microbes
C Xu, Y Zhou, Q Xiao, B He, G Geng, Z Wang, B Cao… - Nature …, 2021 - nature.com
Competitive coevolution between microbes and viruses has led to the diversification of
CRISPR–Cas defense systems against infectious agents. By analyzing metagenomic …
CRISPR–Cas defense systems against infectious agents. By analyzing metagenomic …
SHERLOCK: nucleic acid detection with CRISPR nucleases
Rapid detection of nucleic acids is integral to applications in clinical diagnostics and
biotechnology. We have recently established a CRISPR-based diagnostic platform that …
biotechnology. We have recently established a CRISPR-based diagnostic platform that …
CHOPCHOP v3: expanding the CRISPR web toolbox beyond genome editing
The CRISPR–Cas system is a powerful genome editing tool that functions in a diverse array
of organisms and cell types. The technology was initially developed to induce targeted …
of organisms and cell types. The technology was initially developed to induce targeted …
[HTML][HTML] Development of CRISPR as an antiviral strategy to combat SARS-CoV-2 and influenza
Summary The coronavirus disease 2019 (COVID-19) pandemic, caused by the SARS-CoV-
2 virus, has highlighted the need for antiviral approaches that can target emerging viruses …
2 virus, has highlighted the need for antiviral approaches that can target emerging viruses …
High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral effects
CRISPR–Cas13 systems have recently been used for targeted RNA degradation in various
organisms. However, collateral degradation of bystander RNAs has limited their in vivo …
organisms. However, collateral degradation of bystander RNAs has limited their in vivo …