Adult cortical areas consist of specialized cell types and circuits that support unique higher-
order cognitive functions. How this regional diversity develops from an initially uniform …

The sophisticated circuitry of the neocortex is assembled from a diverse repertoire of
neuronal subtypes generated during development under precise molecular regulation. In …

Spatially resolved transcriptomic technologies are promising tools to study complex
biological processes such as mammalian embryogenesis. However, the imbalance between …

Although the cerebral cortex is organized into six excitatory neuronal layers, it is unclear
whether glial cells show distinct layering. In the present study, we developed a high-content …

Human brain development is underpinned by cellular and molecular reconfigurations
continuing into the third decade of life. To reveal cell dynamics orchestrating neural …

To facilitate scalable profiling of single cells, we developed split-pool ligation-based
transcriptome sequencing (SPLiT-seq), a single-cell RNA-seq (scRNA-seq) method that …

Modeling the processes of neuronal progenitor proliferation and differentiation to produce
mature cortical neuron subtypes is essential for the study of human brain development and …

Here, using further optimized 3D culture that allows highly selective induction and long-term
growth of human ES cell (hESC)-derived cortical neuroepithelium, we demonstrate unique …

Viral infection during pregnancy has been correlated with increased frequency of autism
spectrum disorder (ASD) in offspring. This observation has been modeled in rodents …

Neurons require trophic support during neural circuit formation; however, how the cellular
milieu contributes to neuronal survival remains unclear. We found that layer V cortical …