In eukaryotes, poly (A) tails are present on almost every mRNA. Early experiments led to the
hypothesis that poly (A) tails and the cytoplasmic polyadenylate-binding protein (PABPC) …

The advent of ribosome profiling and other tools to probe mRNA translation has revealed
that codon bias—the uneven use of synonymous codons in the transcriptome—serves as a …

Prime editing enables the installation of virtually any combination of point mutations, small
insertions or small deletions in the DNA of living cells. A prime editing guide RNA (pegRNA) …

Alternative polyadenylation (APA) is an RNA-processing mechanism that generates distinct
3′ termini on mRNAs and other RNA polymerase II transcripts. It is widespread across all …

Summary mRNA degradation represents a critical regulated step in gene expression.
Although the major pathways in turnover have been identified, accounting for disparate half …

Phenotypically identical cells can dramatically vary with respect to behavior during their
lifespan and this variation is reflected in their molecular composition such as the …

mRNA translation decodes nucleotide into amino acid sequences. However, translation has
also been shown to affect mRNA stability depending on codon composition in model …

Protein activity is the ultimate arbiter of function in most cellular pathways, and protein
concentration is fundamentally connected to protein action. While the proteome of yeast has …

An emerging body of evidence indicates that post-transcriptional gene regulation relies not
only on the sequence of mRNAs but also on their folding into intricate secondary structures …

More than half of human genes use alternative cleavage and polyadenylation to generate
alternative 3′ untranslated region (3′ UTR) isoforms. Most efforts have focused on …