[HTML][HTML] Emergent CRISPR–Cas-based technologies for engineering non-model bacteria

DC Volke, E Orsi, PI Nikel - Current Opinion in Microbiology, 2023 - Elsevier
Highlights•CRISPR–Cas systems promoted the engineering of non-model bacteria.•Tools
include genome modifications and finely-tuned transcriptional regulation.•CRISPR–Cas …

Agrobacterium-Mediated Transformation for the Development of Transgenic Crops; Present and Future Prospects

SU Rahman, MO Khan, R Ullah, F Ahmad… - Molecular …, 2024 - Springer
Plant transformation based on Agrobacterium-mediated transformation is a technique that
mimics the natural agrobacterium system for gene (s) introduction into crops. Through this …

From concept to reality: transforming agriculture through innovative rhizosphere engineering for plant health and productivity

MK Solanki, NC Joshi, PK Singh, SK Singh… - Microbiological …, 2023 - Elsevier
The plant rhizosphere is regarded as a microbial hotspot due to a wide array of root
exudates. These root exudates comprise diverse organic compounds such as phenolic …

[HTML][HTML] Identifying Māori perspectives on gene editing in Aotearoa New Zealand

A Clark, P Wilcox, S Morrison, D Munshi… - Communications …, 2024 - nature.com
Māori perspectives on gene technologies are evolving, and traditional cultural constructs
continue to inform a wide diversity of views. Here we summarise a series of research …

Multiplex CRISPR-Cas Genome Editing: Next-Generation Microbial Strain Engineering

SR Lim, SJ Lee - Journal of agricultural and food chemistry, 2024 - ACS Publications
Genome editing is a crucial technology for obtaining desired phenotypes in a variety of
species, ranging from microbes to plants, animals, and humans. With the advent of CRISPR …

[HTML][HTML] Induced Pluripotent Stem Cells and CRISPR-Cas9 Innovations for Treating Alpha-1 Antitrypsin Deficiency and Glycogen Storage Diseases

C Walsh, S Jin - Cells, 2024 - mdpi.com
Human induced pluripotent stem cell (iPSC) and CRISPR-Cas9 gene-editing technologies
have become powerful tools in disease modeling and treatment. By harnessing recent …

Editing microbes to mitigate enteric methane emissions in livestock

FA Khan, A Ali, D Wu, C Huang, H Zulfiqar, M Ali… - World Journal of …, 2024 - Springer
Livestock production significantly contributes to greenhouse gas (GHG) emissions
particularly methane (CH 4) emissions thereby influencing climate change. To address this …

[HTML][HTML] Celebrating the 20th anniversary of the first Xanthomonas genome sequences – how genomics revolutionized taxonomy, provided insight into the …

R Koebnik, S Cesbron, NWG Chen… - Peer Community …, 2024 - peercommunityjournal.org
In this Opinion paper, members of the French Network on Xanthomonads give their personal
view on what they consider to be some of the groundbreaking discoveries in the field of …

Bacterium-enabled transient gene activation by artificial transcription factors for resolving gene regulation in maize

M Zhao, Z Peng, Y Qin, TM Tamang, L Zhang… - The Plant …, 2023 - academic.oup.com
Understanding gene regulatory networks is essential to elucidate developmental processes
and environmental responses. Here, we studied regulation of a maize (Zea mays) …

Single component CRISPR-mediated base-editors for Agrobacterium and their use to develop an improved suite of strains

VJ Pennetti, PR LaFayette, WA Parrott - bioRxiv, 2024 - biorxiv.org
Agrobacterium mediated plant transformation largely depends on two distinct strain lineages-
C58 and Ach5. To better serve the plant transformation community, we have created a suite …