The advent of clustered regularly interspaced short palindromic repeat (CRISPR) has had a
profound impact on plant biology, and crop improvement. In this review, we summarize the …

Unlike CRISPR-Cas9 nucleases, which yield DNA double-strand breaks (DSBs), Cas9
nickases (nCas9s), which are created by replacing key catalytic amino-acid residues in one …

SpCas9 and AsCas12a are widely utilized as genome-editing tools in human cells.
However, their relatively large size poses a limitation for delivery by cargo-size-limited …

Probiotics are the treasure of the microbiology fields. They have been widely used in the
food industry, clinical treatment, and other fields. The equivocal health-promoting effects and …

Cytosine base editors (CBEs) have the potential to correct human pathogenic point
mutations. However, their genome-wide specificity remains poorly understood. Here we …

Dynamic regulation is an effective strategy for control of gene expression in microbial cell
factories. In some pathway contexts, several metabolic modules must be controlled in a time …

Cas12a can process multiple sgRNAs from a single transcript of CRISPR array, conferring
advantages in multiplexed base editing when incorporated into base editor systems, which …

Various genome editing tools have been developed for programmable genome
manipulation at specified genomic loci. However, it is crucial to comprehensively interrogate …

Abstract Background The CRISPR-Cas12a (formerly Cpf1) system is a versatile gene-
editing tool with properties distinct from the broadly used Cas9 system. Features such as …

CRISPR-Cas12a, an RNA-guided DNA targeting endonuclease, has been widely used for
genome editing and nucleic acid detection. As part of the essential processes for both of …