Development of assisted reproductive technologies in small animal species for their efficient preservation and production

K Mochida - Journal of Reproduction and Development, 2020 - jstage.jst.go.jp
Assisted reproductive technologies (ARTs) are widely used in the animal industry, human
clinics, and for basic research. In small laboratory animal species such as mice, ARTs are …

CRISPR/Cas9-based genetic screen of SCNT-reprogramming resistant genes identifies critical genes for male germ cell development in mice

MS Akter, M Hada, D Shikata, G Watanabe, A Ogura… - Scientific Reports, 2021 - nature.com
Male germ cells undergo complex developmental processes eventually producing
spermatozoa through spermatogenesis, although the molecular mechanisms remain largely …

The Effects of Ferulic Acid, Tryptophan, and L-Glutamine on the Cryopreservation of Mouse Spermatozoa

G Koçak, C Yildiz - Biopreservation and Biobanking, 2024 - liebertpub.com
In this study, the effects of ferulic acid (0.1, 1, ve 10 mM), tryptophan (5, 25, ve 50 mM), and L-
glutamine (10, 50, ve 100 mM) at different doses added to 18% raffinose+ 3% skimmed milk …

Easy and quick (EQ) sperm freezing method for urgent preservation of mouse strains

K Mochida, A Hasegawa, D Shikata, N Itami, M Hada… - Scientific Reports, 2021 - nature.com
Cryopreservation of mouse spermatozoa is widely used for the efficient preservation and
safe transport of valuable mouse strains. However, the current cryopreservation method …

Improvement of short straws for sperm cryopreservation: installing an air-permeable filter facilitates handling

R Kaneko, T Kakinuma, S Sato… - Journal of Reproduction …, 2021 - jstage.jst.go.jp
Saving space for sperm cryopreservation would aid mouse genetics research. We
previously developed the ST (sperm freezing in ShorT STraw to reduce STorage space) …

Effect of D-aspartate and storage temperature on cryopreserved sperm quality and fertility in mice

M Peltier - 2024 - kups.ub.uni-koeln.de
Recent advances in genetic engineering have resulted in the generation of thousands of
transgenic mouse lines. Assisted reproductive technologies have become essential for …