Cryo‐electron tomography (cryo‐ET) provides high‐resolution 3D views into cells pristinely
preserved by vitrification. Recent technical advances such as direct electron detectors, the …

The bacterial division apparatus catalyses the synthesis and remodelling of septal
peptidoglycan (sPG) to build the cell wall layer that fortifies the daughter cell poles …

Recent advances have made cryogenic (cryo) electron microscopy a key technique to
achieve near-atomic-resolution structures of biochemically isolated macromolecular …

After gradually moving away from preparation methods prone to artefacts such as plastic
embedding and negative staining for cell sections and single particles, the field of cryo …

The increasing demand for cryo-electron microscopy (cryo-EM) reveals drawbacks in current
sample preparation protocols, such as sample waste and lack of reproducibility. Here, we …

Cryo-electron tomography (cryo-ET) has become a powerful approach to study the high-
resolution structure of cellular macromolecular machines in situ. However, the current …

Cryo-electron tomography (cryo-ET) is emerging as a revolutionary method for resolving the
structure of macromolecular complexes in situ. However, sample preparation for in situ Cryo …

Cryo-electron tomography (cryoET) has become a powerful technique at the interface of
structural biology and cell biology, due to its unique ability for imaging cells in their native …

Microvilli are actin-bundle-supported membrane protrusions essential for absorption,
secretion, and sensation. Microvilli defects cause gastrointestinal disorders; however …

The introduction of cryo‐techniques to the focused ion‐beam scanning electron microscope
(FIB‐SEM) has brought new opportunities to study frozen, hydrated samples from the field of …