Dynamic imaging of nascent RNA reveals general principles of transcription dynamics and stochastic splice site selection
The activities of RNA polymerase and the spliceosome are responsible for the heterogeneity
in the abundance and isoform composition of mRNA in human cells. However, the dynamics …
in the abundance and isoform composition of mRNA in human cells. However, the dynamics …
Real-time imaging of a single gene reveals transcription-initiated local confinement
Genome dynamics are intimately linked to the regulation of gene expression, the most
fundamental mechanism in biology, yet we still do not know whether the very process of …
fundamental mechanism in biology, yet we still do not know whether the very process of …
A ubiquitous disordered protein interaction module orchestrates transcription elongation
K Cermakova, J Demeulemeester, V Lux, M Nedomova… - Science, 2021 - science.org
During eukaryotic transcription elongation, RNA polymerase II (RNAP2) is regulated by a
chorus of factors. Here, we identified a common binary interaction module consisting of TFIIS …
chorus of factors. Here, we identified a common binary interaction module consisting of TFIIS …
Overview of CDK9 as a target in cancer research
F Morales, A Giordano - Cell Cycle, 2016 - Taylor & Francis
ABSTRACT CDK9 is a protein in constant development in cancer therapy. Herein we
present an overview of the enzyme as a target for cancer therapy. We provide data on its …
present an overview of the enzyme as a target for cancer therapy. We provide data on its …
DNA methylation directs microRNA biogenesis in mammalian cells
MicroRNA (miRNA) biogenesis initiates co-transcriptionally, but how the Microprocessor
machinery pinpoints the locations of short precursor miRNA sequences within long flanking …
machinery pinpoints the locations of short precursor miRNA sequences within long flanking …
The pausing zone and control of RNA polymerase II elongation by Spt5: Implications for the pause-release model
The pause-release model of transcription proposes that 40–100 bases from the start site
RNA Pol II pauses, followed by release into productive elongation. Pause release is …
RNA Pol II pauses, followed by release into productive elongation. Pause release is …
Alternative RNA structures formed during transcription depend on elongation rate and modify RNA processing
T Saldi, K Riemondy, B Erickson, DL Bentley - Molecular cell, 2021 - cell.com
Most RNA processing occurs co-transcriptionally. We interrogated nascent pol II transcripts
by chemical and enzymatic probing and determined how the" nascent RNA structureome" …
by chemical and enzymatic probing and determined how the" nascent RNA structureome" …
Transcriptional control by premature termination: a forgotten mechanism
K Kamieniarz-Gdula, NJ Proudfoot - Trends in Genetics, 2019 - cell.com
The concept of early termination as an important means of transcriptional control has long
been established. Even so, its role in metazoan gene expression is underappreciated …
been established. Even so, its role in metazoan gene expression is underappreciated …
Profiling lariat intermediates reveals genetic determinants of early and late co-transcriptional splicing
Long introns with short exons in vertebrate genes are thought to require spliceosome
assembly across exons (exon definition), rather than introns, thereby requiring transcription …
assembly across exons (exon definition), rather than introns, thereby requiring transcription …
RNA polymerase II clusters form in line with surface condensation on regulatory chromatin
It is essential for cells to control which genes are transcribed into RNA. In eukaryotes, two
major control points are recruitment of RNA polymerase II (Pol II) into a paused state, and …
major control points are recruitment of RNA polymerase II (Pol II) into a paused state, and …