Transforming the vast knowledge from genetics, biochemistry, and structural biology into
detailed molecular descriptions of biological processes inside cells remains a major …

Release of promoter-proximal paused RNA polymerase II (Pol II) during early elongation is a
critical step in transcriptional regulation in metazoan cells. Paused Pol II release is thought …

Transcription of eukaryotic genes by RNA polymerase II (Pol II) yields RNA precursors
containing introns that must be spliced out and the flanking exons ligated together. Splicing …

The transcription cycle can be roughly divided into three stages: initiation, elongation, and
termination. Understanding the molecular events that regulate all these stages requires a …

Abstract Cyclin-dependent kinase 12 (CDK12) phosphorylates the carboxyl-terminal domain
(CTD) of RNA polymerase II (pol II) but its roles in transcription beyond the expression of …

Mutations in one SETD5 allele are genetic causes of intellectual disability and autistic
spectrum disorders. However, the mechanisms by which SETD5 regulates brain …

Multi-dimensional omics profiling continues to illuminate the complexity of cellular
processes. Because of difficult mechanistic interpretation of phenotypes induced by slow …

The remarkable capacity for pluripotency and self-renewal in embryonic stem cells (ESCs)
requires a finely tuned transcriptional circuitry wherein the pathways and genes that initiate …

Transcriptional regulation occurs via changes to rates of different biochemical steps of
transcription, but it remains unclear which rates are subject to change upon biological …

Transcription controls splicing and other gene regulatory processes, yet mechanisms remain
obscure due to our fragmented knowledge of the molecular connections between the …