Identification of reliable reference genes for quantitative real-time PCR normalization in pitaya

C Chen, J Wu, Q Hua, N Tel-Zur, F Xie, Z Zhang… - Plant Methods, 2019 - Springer
Background A suitable reference gene is an important prerequisite for guarantying accurate
and reliable results in quantitative real-time PCR (qRT-PCR) analyses. However, there is no …

Identification and Validation of Reference Genes for Gene Expression Analysis in Schima superba

Z Yang, R Zhang, Z Zhou - Genes, 2021 - mdpi.com
Real-time quantitative PCR (RT-qPCR) is a reliable and high-throughput technique for gene
expression studies, but its accuracy depends on the expression stability of reference genes …

Insight into Catechins Metabolic Pathways of Camellia sinensis Based on Genome and Transcriptome Analysis

W Wang, Y Zhou, Y Wu, X Dai, Y Liu… - Journal of agricultural …, 2018 - ACS Publications
Tea is an important economic crop with a 3.02 Gb genome. It accumulates various bioactive
compounds, especially catechins, which are closely associated with tea flavor and quality …

Identification and characterization of two sesquiterpene synthase genes involved in volatile-mediated defense in tea plant (Camellia sinensis)

G Liu, M Yang, J Fu - Plant Physiology and Biochemistry, 2020 - Elsevier
Terpenes and their derivatives are vital components of tea aroma. Their constitution and
quantity are highly important criteria for the sensory evaluation of teas. Biologically, terpenes …

Reliable reference genes for normalization of gene expression data in tea plants (Camellia sinensis) exposed to metal stresses

ML Wang, QH Li, HH Xin, X Chen, XJ Zhu, XH Li - PloS one, 2017 - journals.plos.org
Tea plants [Camellia sinensis (L.) O. Kuntze] are an important leaf-type crop that are widely
used for the production of non-alcoholic beverages in the world. Exposure to excessive …

Molecular cloning, functional analysis of three cinnamyl alcohol dehydrogenase (CAD) genes in the leaves of tea plant, Camellia sinensis

WW Deng, M Zhang, JQ Wu, ZZ Jiang, L Tang… - Journal of plant …, 2013 - Elsevier
Cinnamyl alcohol dehydrogenase (CAD; EC 1.1. 1.195) is considered to be a key enzyme in
lignin biosynthesis, but little was known about CADs in tea plants (Camellia sinensis). A full …

Isolation and functional analysis of squalene synthase gene in tea plant Camellia sinensis

J Fu, G Liu, M Yang, X Wang, X Chen, F Chen… - Plant Physiology and …, 2019 - Elsevier
Tea contains high quantities and diverse types of triterpenoids, particularly in the form of
saponins. However, little is yet known about the molecular basis of triterpenoid biosynthesis …

Evaluation and selection of suitable qRT-PCR reference genes for light responses in tea plant (Camellia sinensis)

J Wang, L Liu, Q Tang, K Sun, L Zeng, Z Wu - Scientia Horticulturae, 2021 - Elsevier
Tea plant (Camellia sinensis) is an important woody economic crop used for processing leaf-
type beverages. Numerous studies have shown that light is a necessary environmental …

Identification and evaluation of reliable reference genes for quantitative real-time PCR analysis in tea plants under differential biotic stresses

W Xu, Y Dong, Y Yu, Y Xing, X Li, X Zhang, X Hou… - Scientific Reports, 2020 - nature.com
The selection of reliable reference genes (RGs) for normalization under given experimental
conditions is necessary to develop an accurate qRT-PCR assay. To the best of our …

Evaluation of candidate reference genes for normalization of quantitative RT-PCR in switchgrass under various abiotic stress conditions

L Huang, H Yan, X Jiang, X Zhang, Y Zhang… - BioEnergy …, 2014 - Springer
Quantitative real-time reverse transcriptase PCR (qRT-PCR) is a sensitive and powerful
technique for measuring differential gene expression; however, changes in gene expression …