The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-
associated protein 9 (Cas9) system has become the most important tool for targeted genome …

Molecular cloning is a cornerstone of biomedical, biotechnological, and synthetic biology
research. As such, improved cloning methodologies can significantly advance the speed …

Dynamic measurements of molecular machines can provide invaluable insights into their
mechanism, but these measurements have been challenging in living cells. Here, we …

The assembly of DNA fragments with homologous arms is becoming popular in routine
cloning. For an in vitro assembly reaction, a DNA polymerase is often used either alone for …

Optogenetics is the genetic approach for controlling cellular processes with light. It provides
spatiotemporal, quantitative and reversible control over biological signaling and metabolic …

Light-inducible gene switches represent a key strategy for the precise manipulation of
cellular events in fundamental and applied research. However, the performance of widely …

In vivo homologous recombination holds the potential for optimal molecular cloning,
however, current strategies require specialised bacterial strains or laborious protocols. Here …

Golden Gate cloning is a prominent DNA assembly tool in synthetic biology for the assembly
of plasmid constructs often used in combinatorial pathway optimization, with a number of …

Cytochrome bd quinol: O2 oxidoreductases are respiratory terminal oxidases so far only
identified in prokaryotes, including several pathogenic bacteria. Escherichia coli contains …

High‐efficiency methods for DNA assembly have enabled the routine assembly of synthetic
DNA s of increased size and complexity. However, these techniques require customization …