Live-dead assay on unlabeled cells using phase imaging with computational specificity

C Hu, S He, YJ Lee, Y He, EM Kong, H Li… - Nature …, 2022 - nature.com
Existing approaches to evaluate cell viability involve cell staining with chemical reagents.
However, the step of exogenous staining makes these methods undesirable for rapid …

Machine learning assisted classification of cell lines and cell states on quantitative phase images

AV Belashov, AA Zhikhoreva, TN Belyaeva, AV Salova… - Cells, 2021 - mdpi.com
In this report, we present implementation and validation of machine-learning classifiers for
distinguishing between cell types (HeLa, A549, 3T3 cell lines) and states (live, necrosis …

Investigating fibroblast cells under “safe” and “injurious” blue‐light exposure by holographic microscopy

A Calabuig, M Mugnano, L Miccio, S Grilli… - Journal of …, 2017 - Wiley Online Library
The exposure to visible light has been shown to exert various biological effects, such as
erythema and retinal degeneration. However, the phototoxicity mechanisms in living cells …

In vitro cytotoxicity evaluation of cadmium by label‐free holographic microscopy

M Mugnano, P Memmolo, L Miccio, S Grilli… - Journal of …, 2018 - Wiley Online Library
Among all environmental pollutants, the toxic heavy metal cadmium is considered as a
human carcinogen. Cadmium may induce cell death by apoptosis in various cell types …

Label‐free high temporal resolution assessment of cell proliferation using digital holographic microscopy

B Janicke, A Kårsnäs, P Egelberg, K Alm - Cytometry Part A, 2017 - Wiley Online Library
Cell proliferation assays are widely applied in biological sciences to understand the effect of
drugs over time. However, current methods often assess cell population growth indirectly …

Novel optical approaches for label-free quantification of nano-cytotoxic effects

S Mues, J Antunovic, S Ketelhut… - Biophysics, Biology …, 2016 - spiedigitallibrary.org
Commonly used cytotoxicity assays to determine the formation of reactive oxygen species,
cell viability or cell death are often affected by applied nanomaterials, which lead to false …

Digital holographic microscopy as multimodal read out for in vitro nanomaterial cytotoxicity testing

S Mues, S Ketelhut, B Kemper… - … on Biomedical Optics, 2017 - opg.optica.org
Digital holographic microscopy as multimodal read out for in vitro nanomaterial cytotoxicity
testing Page 1 Digital holographic microscopy as multimodal read out for in vitro …

Optical analysis of nanomaterial-cell interactions: Flow cytometry and digital holographic microscopy

S Mues, J Antunovic, R Ossig… - Optical Methods for …, 2015 - spiedigitallibrary.org
The in vitro cytotoxicity assessment of engineered nanoparticles commonly involves the
measurement of different endpoints like the formation of reactive oxygen species, cell …

Digital holographic microscopy overcomes the limitations of in vitro nanomaterial cytotoxicity testing

S Mues, S Ketelhut, B Kemper… - … Phase Imaging III, 2017 - spiedigitallibrary.org
The cytotoxic potential of nanomaterials is commonly evaluated by different cellular
endpoints as reactive oxygen species formation, cell viability or cell death. Usually these …

Testing of bioactive-nanovesicles on hepatotoxicity of atypical antipsychotics via digital holography

FO Kirbay, C Geyik, E Guler, O Yesiltepe… - Colloids and Surfaces B …, 2017 - Elsevier
Atypical antipsychotic drugs induce hepatic toxicity. Thus, it is of importance to eliminate the
side effects of these drugs. Herein we describe the preparation of nanoemulsions with a …