Chloroplast ATP synthase biogenesis requires peripheral stalk subunits AtpF and ATPG and stabilization of atpE mRNA by OPR protein MDE1

F Chaux, D Jarrige, M Rodrigues‐Azevedo… - The Plant …, 2023 - Wiley Online Library
Chloroplast ATP synthase contains subunits of plastid and nuclear genetic origin. To
investigate the coordinated biogenesis of this complex, we isolated novel ATP synthase …

[HTML][HTML] CRISPR-based bioengineering in microalgae for production of industrially important biomolecules

D Dhokane, A Shaikh, A Yadav, N Giri… - Frontiers in …, 2023 - ncbi.nlm.nih.gov
Microalgae, as photosynthetic organisms, have the potential to produce biomolecules for
use in food, feed, cosmetics, nutraceuticals, fuel, and other applications. Faster growth rates …

Chlamydomonas POLQ is necessary for CRISPR/Cas9-mediated gene targeting

I Sizova, S Kelterborn, V Verbenko, S Kateriya… - G3, 2021 - academic.oup.com
The use of CRISPR/Cas endonucleases has revolutionized gene editing techniques for
research on Chlamydomonas reinhardtii. To better utilize the CRISPR/Cas system, it is …

Co-targeting strategy for precise, scarless gene editing with CRISPR/Cas9 and donor ssODNs in Chlamydomonas

S Akella, X Ma, R Bacova, ZP Harmer… - Plant …, 2021 - academic.oup.com
Programmable site-specific nucleases, such as the clustered regularly interspaced short
palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) ribonucleoproteins …

BE3 is the major branching enzyme isoform required for amylopectin synthesis in Chlamydomonas reinhardtii

A Courseaux, O George, P Deschamps… - Frontiers in Plant …, 2023 - frontiersin.org
Starch-branching enzymes (BEs) are essential for starch synthesis in both plants and algae
where they influence the architecture and physical properties of starch granules. Within …

Genome Editing in Chlamydomonas reinhardtii Using Cas9-gRNA Ribonucleoprotein Complex: A Step-by-Step Guide

D Dhokane, N Kancharla, A Savarimuthu… - … : Methods and Protocols, 2023 - Springer
Genome editing technologies have provided opportunities to manipulate literally any
genomic location, opening new avenues for reverse genetics-based improvements. Among …

High‐throughput identification of novel heat tolerance genes via genome‐wide pooled mutant screens in the model green alga Chlamydomonas reinhardtii

EM Mattoon, WE McHargue, CE Bailey… - Plant, cell & …, 2023 - Wiley Online Library
Different high temperatures adversely affect crop and algal yields with various responses in
photosynthetic cells. The list of genes required for thermotolerance remains elusive …

Moderate high temperature is beneficial or detrimental depending on carbon availability in the green alga Chlamydomonas reinhardtii

N Zhang, B Venn, CE Bailey, M Xia… - Journal of …, 2024 - academic.oup.com
High temperatures impair plant growth and reduce agricultural yields, but the underlying
mechanisms remain elusive. The unicellular green alga Chlamydomonas reinhardtii is an …

Comparative Phenotyping of Two Commonly Used Chlamydomonas reinhardtii Background Strains: CC-1690 (21gr) and CC-5325 (The CLiP Mutant Library …

N Zhang, L Pazouki, H Nguyen, S Jacobshagen… - Plants, 2022 - mdpi.com
The unicellular green alga Chlamydomonas reinhardtii is an excellent model organism to
investigate many essential cellular processes in photosynthetic eukaryotes. Two commonly …

CRISPR/Cas 基因编辑技术及其在微藻研究中的应用

陈映丹, 张扬, 夏嫱, 孙虹霞 - 生物技术通报, 2022 - biotech.aiijournal.com
微藻由于在医药, 食品, 可再生燃料和化学原料等方面的潜力, 受到了研究者越来越多的关注和
青睐. 然而, 合适基因编辑方法和转化工具的缺乏, 使得微藻基因工程的进展还相对比较缓慢 …