Chloroplast ATP synthase biogenesis requires peripheral stalk subunits AtpF and ATPG and stabilization of atpE mRNA by OPR protein MDE1
F Chaux, D Jarrige, M Rodrigues‐Azevedo… - The Plant …, 2023 - Wiley Online Library
Chloroplast ATP synthase contains subunits of plastid and nuclear genetic origin. To
investigate the coordinated biogenesis of this complex, we isolated novel ATP synthase …
investigate the coordinated biogenesis of this complex, we isolated novel ATP synthase …
[HTML][HTML] CRISPR-based bioengineering in microalgae for production of industrially important biomolecules
D Dhokane, A Shaikh, A Yadav, N Giri… - Frontiers in …, 2023 - ncbi.nlm.nih.gov
Microalgae, as photosynthetic organisms, have the potential to produce biomolecules for
use in food, feed, cosmetics, nutraceuticals, fuel, and other applications. Faster growth rates …
use in food, feed, cosmetics, nutraceuticals, fuel, and other applications. Faster growth rates …
Chlamydomonas POLQ is necessary for CRISPR/Cas9-mediated gene targeting
I Sizova, S Kelterborn, V Verbenko, S Kateriya… - G3, 2021 - academic.oup.com
The use of CRISPR/Cas endonucleases has revolutionized gene editing techniques for
research on Chlamydomonas reinhardtii. To better utilize the CRISPR/Cas system, it is …
research on Chlamydomonas reinhardtii. To better utilize the CRISPR/Cas system, it is …
Co-targeting strategy for precise, scarless gene editing with CRISPR/Cas9 and donor ssODNs in Chlamydomonas
S Akella, X Ma, R Bacova, ZP Harmer… - Plant …, 2021 - academic.oup.com
Programmable site-specific nucleases, such as the clustered regularly interspaced short
palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) ribonucleoproteins …
palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) ribonucleoproteins …
BE3 is the major branching enzyme isoform required for amylopectin synthesis in Chlamydomonas reinhardtii
A Courseaux, O George, P Deschamps… - Frontiers in Plant …, 2023 - frontiersin.org
Starch-branching enzymes (BEs) are essential for starch synthesis in both plants and algae
where they influence the architecture and physical properties of starch granules. Within …
where they influence the architecture and physical properties of starch granules. Within …
Genome Editing in Chlamydomonas reinhardtii Using Cas9-gRNA Ribonucleoprotein Complex: A Step-by-Step Guide
D Dhokane, N Kancharla, A Savarimuthu… - … : Methods and Protocols, 2023 - Springer
Genome editing technologies have provided opportunities to manipulate literally any
genomic location, opening new avenues for reverse genetics-based improvements. Among …
genomic location, opening new avenues for reverse genetics-based improvements. Among …
High‐throughput identification of novel heat tolerance genes via genome‐wide pooled mutant screens in the model green alga Chlamydomonas reinhardtii
EM Mattoon, WE McHargue, CE Bailey… - Plant, cell & …, 2023 - Wiley Online Library
Different high temperatures adversely affect crop and algal yields with various responses in
photosynthetic cells. The list of genes required for thermotolerance remains elusive …
photosynthetic cells. The list of genes required for thermotolerance remains elusive …
Moderate high temperature is beneficial or detrimental depending on carbon availability in the green alga Chlamydomonas reinhardtii
High temperatures impair plant growth and reduce agricultural yields, but the underlying
mechanisms remain elusive. The unicellular green alga Chlamydomonas reinhardtii is an …
mechanisms remain elusive. The unicellular green alga Chlamydomonas reinhardtii is an …
Comparative Phenotyping of Two Commonly Used Chlamydomonas reinhardtii Background Strains: CC-1690 (21gr) and CC-5325 (The CLiP Mutant Library …
The unicellular green alga Chlamydomonas reinhardtii is an excellent model organism to
investigate many essential cellular processes in photosynthetic eukaryotes. Two commonly …
investigate many essential cellular processes in photosynthetic eukaryotes. Two commonly …
CRISPR/Cas 基因编辑技术及其在微藻研究中的应用
陈映丹, 张扬, 夏嫱, 孙虹霞 - 生物技术通报, 2022 - biotech.aiijournal.com
微藻由于在医药, 食品, 可再生燃料和化学原料等方面的潜力, 受到了研究者越来越多的关注和
青睐. 然而, 合适基因编辑方法和转化工具的缺乏, 使得微藻基因工程的进展还相对比较缓慢 …
青睐. 然而, 合适基因编辑方法和转化工具的缺乏, 使得微藻基因工程的进展还相对比较缓慢 …