[HTML][HTML] Human organoids in basic research and clinical applications

XY Tang, S Wu, D Wang, C Chu, Y Hong… - Signal transduction and …, 2022 - nature.com
Organoids are three-dimensional (3D) miniature structures cultured in vitro produced from
either human pluripotent stem cells (hPSCs) or adult stem cells (AdSCs) derived from …

[HTML][HTML] Towards organoid culture without Matrigel

MT Kozlowski, CJ Crook, HT Ku - Communications biology, 2021 - nature.com
Organoids—cellular aggregates derived from stem or progenitor cells that recapitulate organ
function in miniature—are of growing interest in developmental biology and medicine …

Culture and analysis of kidney tubuloids and perfused tubuloid cells-on-a-chip

L Gijzen, FA Yousef Yengej, F Schutgens… - Nature protocols, 2021 - nature.com
Advanced in vitro kidney models are of great importance to the study of renal physiology and
disease. Kidney tubuloids can be established from primary cells derived from adult kidney …

Tubuloid culture enables long-term expansion of functional human kidney tubule epithelium from iPSC-derived organoids

FA Yousef Yengej, J Jansen… - Proceedings of the …, 2023 - National Acad Sciences
Kidney organoids generated from induced pluripotent stem cells (iPSC) have proven
valuable for studies of kidney development, disease, and therapeutic screening. However …

Recent advances in three‐dimensional stem cell culture systems and applications

X Wu, J Su, J Wei, N Jiang, X Ge - Stem cells international, 2021 - Wiley Online Library
Cell culture is one of the most core and fundamental techniques employed in the fields of
biology and medicine. At present, although the two‐dimensional cell culture method is …

Stem cells for next level toxicity testing in the 21st century

E Fritsche, T Haarmann‐Stemmann, J Kapr, S Galanjuk… - Small, 2021 - Wiley Online Library
The call for a paradigm change in toxicology from the United States National Research
Council in 2007 initiates awareness for the invention and use of human‐relevant alternative …

Gastrointestinal organs and organoids-on-a-chip: advances and translation into the clinics

MR Carvalho, LP Yan, B Li, CH Zhang, YL He… - …, 2023 - iopscience.iop.org
Microfluidic organs and organoids-on-a-chip models of human gastrointestinal systems
have been established to recreate adequate microenvironments to study physiology and …

Soft, dynamic hydrogel confinement improves kidney organoid lumen morphology and reduces epithelial–mesenchymal transition in culture

FAA Ruiter, FLC Morgan, N Roumans… - Advanced …, 2022 - Wiley Online Library
Pluripotent stem cell‐derived kidney organoids offer a promising solution to renal failure, yet
current organoid protocols often lead to off‐target cells and phenotypic alterations …

Transformative materials to create 3D functional human tissue models in vitro in a reproducible manner

JL Gerardo‐Nava, J Jansen, D Günther… - Advanced …, 2023 - Wiley Online Library
Recreating human tissues and organs in the petri dish to establish models as tools in
biomedical sciences has gained momentum. These models can provide insight into …

[HTML][HTML] Tubuloid differentiation to model the human distal nephron and collecting duct in health and disease

FAY Yengej, CP Casellas, CME Ammerlaan… - Cell Reports, 2024 - cell.com
Organoid technology is rapidly gaining ground for studies on organ (patho) physiology.
Tubuloids are long-term expanding organoids grown from adult kidney tissue or urine. The …