[HTML][HTML] scFv antibody: principles and clinical application
To date, generation of single-chain fragment variable (scFv) has become an established
technique used to produce a completely functional antigen-binding fragment in bacterial …
technique used to produce a completely functional antigen-binding fragment in bacterial …
[HTML][HTML] Targeted intracellular delivery of antibodies: the state of the art
A dominant area of antibody research is the extension of the use of this mighty experimental
and therapeutic tool for the specific detection of molecules for diagnostics, visualization, and …
and therapeutic tool for the specific detection of molecules for diagnostics, visualization, and …
Production of active eukaryotic proteins through bacterial expression systems: a review of the existing biotechnology strategies
S Sahdev, SK Khattar, KS Saini - Molecular and cellular biochemistry, 2008 - Springer
Among the various expression systems employed for the over-production of proteins,
bacteria still remains the favorite choice of a Protein Biochemist. However, even today, due …
bacteria still remains the favorite choice of a Protein Biochemist. However, even today, due …
[HTML][HTML] Strategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coli
A De Marco - Microbial cell factories, 2009 - Springer
Bacteria are simple and cost effective hosts for producing recombinant proteins. However,
their physiological features may limit their use for obtaining in native form proteins of some …
their physiological features may limit their use for obtaining in native form proteins of some …
[HTML][HTML] Thioredoxins and glutaredoxins as facilitators of protein folding
C Berndt, CH Lillig, A Holmgren - … et biophysica acta (BBA)-molecular cell …, 2008 - Elsevier
Thiol–disulfide oxidoreductase systems of bacterial cytoplasm and eukaryotic cytosol favor
reducing conditions and protein thiol groups, while bacterial periplasm and eukaryotic …
reducing conditions and protein thiol groups, while bacterial periplasm and eukaryotic …
Targeted delivery of anti-CD19 liposomal doxorubicin in B-cell lymphoma: a comparison of whole monoclonal antibody, Fab′ fragments and single chain Fv
WWK Cheng, TM Allen - Journal of Controlled Release, 2008 - Elsevier
As part of an ongoing effort to develop a clinically acceptable doxorubicin formulation,
targeted against B-cell malignancies, this study compared long-circulating (Stealth®) …
targeted against B-cell malignancies, this study compared long-circulating (Stealth®) …
Screening of fusion partners for high yield expression and purification of bioactive viscotoxins
Viscotoxins are small cationic proteins found in European mistletoe Viscum album. They are
highly toxic towards phytopathogenic fungi and cancer cells. Heterologous expression of …
highly toxic towards phytopathogenic fungi and cancer cells. Heterologous expression of …
[HTML][HTML] Mitochondrial thioredoxin system is required for enhanced stress resistance and extended longevity in long-lived mitochondrial mutants
Mild impairment of mitochondrial function has been shown to increase lifespan in genetic
model organisms including worms, flies and mice. To better understand the mechanisms …
model organisms including worms, flies and mice. To better understand the mechanisms …
[HTML][HTML] Evolutionarily conserved role of thioredoxin systems in determining longevity
A AlOkda, JM Van Raamsdonk - Antioxidants, 2023 - mdpi.com
Thioredoxin and thioredoxin reductase are evolutionarily conserved antioxidant enzymes
that protect organisms from oxidative stress. These proteins also play roles in redox …
that protect organisms from oxidative stress. These proteins also play roles in redox …
Multivalency: the hallmark of antibodies used for optimization of tumor targeting by design
SM Deyev, EN Lebedenko - Bioessays, 2008 - Wiley Online Library
High‐precision tumor targeting with conventional therapeutics is based on the concept of the
ideal drug as a “magic bullet”; this became possible after techniques were developed for …
ideal drug as a “magic bullet”; this became possible after techniques were developed for …