[HTML][HTML] scFv antibody: principles and clinical application

ZA Ahmad, SK Yeap, AM Ali, WY Ho… - Journal of Immunology …, 2012 - hindawi.com
To date, generation of single-chain fragment variable (scFv) has become an established
technique used to produce a completely functional antigen-binding fragment in bacterial …

[HTML][HTML] Targeted intracellular delivery of antibodies: the state of the art

TA Slastnikova, AV Ulasov, AA Rosenkranz… - Frontiers in …, 2018 - frontiersin.org
A dominant area of antibody research is the extension of the use of this mighty experimental
and therapeutic tool for the specific detection of molecules for diagnostics, visualization, and …

Production of active eukaryotic proteins through bacterial expression systems: a review of the existing biotechnology strategies

S Sahdev, SK Khattar, KS Saini - Molecular and cellular biochemistry, 2008 - Springer
Among the various expression systems employed for the over-production of proteins,
bacteria still remains the favorite choice of a Protein Biochemist. However, even today, due …

[HTML][HTML] Strategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coli

A De Marco - Microbial cell factories, 2009 - Springer
Bacteria are simple and cost effective hosts for producing recombinant proteins. However,
their physiological features may limit their use for obtaining in native form proteins of some …

[HTML][HTML] Thioredoxins and glutaredoxins as facilitators of protein folding

C Berndt, CH Lillig, A Holmgren - … et biophysica acta (BBA)-molecular cell …, 2008 - Elsevier
Thiol–disulfide oxidoreductase systems of bacterial cytoplasm and eukaryotic cytosol favor
reducing conditions and protein thiol groups, while bacterial periplasm and eukaryotic …

Targeted delivery of anti-CD19 liposomal doxorubicin in B-cell lymphoma: a comparison of whole monoclonal antibody, Fab′ fragments and single chain Fv

WWK Cheng, TM Allen - Journal of Controlled Release, 2008 - Elsevier
As part of an ongoing effort to develop a clinically acceptable doxorubicin formulation,
targeted against B-cell malignancies, this study compared long-circulating (Stealth®) …

Screening of fusion partners for high yield expression and purification of bioactive viscotoxins

J Bogomolovas, B Simon, M Sattler, G Stier - Protein expression and …, 2009 - Elsevier
Viscotoxins are small cationic proteins found in European mistletoe Viscum album. They are
highly toxic towards phytopathogenic fungi and cancer cells. Heterologous expression of …

[HTML][HTML] Mitochondrial thioredoxin system is required for enhanced stress resistance and extended longevity in long-lived mitochondrial mutants

N Harris-Gauthier, A Traa, A AlOkda, A Moldakozhayev… - Redox biology, 2022 - Elsevier
Mild impairment of mitochondrial function has been shown to increase lifespan in genetic
model organisms including worms, flies and mice. To better understand the mechanisms …

[HTML][HTML] Evolutionarily conserved role of thioredoxin systems in determining longevity

A AlOkda, JM Van Raamsdonk - Antioxidants, 2023 - mdpi.com
Thioredoxin and thioredoxin reductase are evolutionarily conserved antioxidant enzymes
that protect organisms from oxidative stress. These proteins also play roles in redox …

Multivalency: the hallmark of antibodies used for optimization of tumor targeting by design

SM Deyev, EN Lebedenko - Bioessays, 2008 - Wiley Online Library
High‐precision tumor targeting with conventional therapeutics is based on the concept of the
ideal drug as a “magic bullet”; this became possible after techniques were developed for …